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  1. Link

    alpha-1-antitrypsin-antibody-ab9373.pdf

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Cardiovascular Blood Serum Proteins
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Anti-alpha 1 Antitrypsin 抗体 (ab9373)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (ab9373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)

Key features and details

  • Rabbit polyclonal to alpha 1 Antitrypsin
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-alpha 1 Antitrypsin antibody
    alpha 1 Antitrypsin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to alpha 1 Antitrypsin
  • 由来種

    Rabbit
  • 特異性

    The antibody is specific against human alpha-1-antitrypsin.
  • アプリケーション

    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Human alpha 1 Antitrypsin. Isolated from human serum.
    Database link: P01009

  • ポジティブ・コントロール

    • IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 軽鎖の種類

    unknown
  • 研究分野

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human alpha 1 Antitrypsin protein (ab79181)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9373の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.
特記事項
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 組織特異性

    Ubiquitous. Expressed in leukocytes and plasma.
  • 関連疾患

    Alpha-1-antitrypsin deficiency
  • 配列類似性

    Belongs to the serpin family.
  • ドメイン

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻訳後修飾

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 細胞内局在

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P01009 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5265 Human
    • Omim: 107400 Human
    • SwissProt: P01009 Human
    • Unigene: 525557 Human
    • 別名

      • A1A antibody
      • A1AT antibody
      • A1AT_HUMAN antibody
      • AAT antibody
      • Alpha 1 antiproteinase antibody
      • Alpha 1 antitrypsin antibody
      • Alpha 1 antitrypsin null antibody
      • Alpha 1 protease inhibitor antibody
      • Alpha-1 protease inhibitor antibody
      • Alpha-1-antiproteinase antibody
      • alpha1 proteinase inhibitor antibody
      • Alpha1AT antibody
      • Dom1 antibody
      • PI antibody
      • PI1 antibody
      • PRO0684 antibody
      • PRO2275 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
      • Serine protease inhibitor 1-1 antibody
      • Serine protease inhibitor A1a antibody
      • Serpin A1 antibody
      • Serpin A1a antibody
      • Serpin peptidase inhibitor clade A member 1 antibody
      • Serpina1 antibody
      • Short peptide from AAT antibody
      • SPAAT antibody
      • Spi1-1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (ab9373)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (ab9373)

      ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
      ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (9)

    ab9373 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9373 は 9 報の論文で使用されています。

    • Smith SM & Melrose J A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-a-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface. Int J Mol Sci 20:N/A (2019). PubMed: 30678366
    • Skurikhin EG  et al. Endothelial Progenitor Cells as Pathogenetic and Diagnostic Factors, and Potential Targets for GLP-1 in Combination with Metabolic Syndrome and Chronic Obstructive Pulmonary Disease. Int J Mol Sci 20:N/A (2019). PubMed: 30836679
    • Tang W  et al. Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor. Acta Pharmacol Sin 40:620-629 (2019). PubMed: 30315254
    • Mun SJ  et al. Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids. J Hepatol 71:970-985 (2019). PubMed: 31299272
    • Ookawa S  et al. Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma. World Neurosurg 111:e783-e789 (2018). PubMed: 29309973
    • Laohaviroj M  et al. A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma. Tumour Biol 39:1010428317705764 (2017). PubMed: 28618946
    • Jacobo SM  et al. Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1. Mol Cell Biol 33:1976-90 (2013). PubMed: 23478260
    • Kamimura K  et al. Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver. Mol Ther Nucleic Acids 2:e128 (2013). IHC-P ; Human . PubMed: 24129227
    • Kamimura K  et al. Image-guided, intravascular hydrodynamic gene delivery to skeletal muscle in pigs. Mol Ther 18:93-100 (2010). IHC-P ; Human . PubMed: 19738603

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-alpha 1 Antitrypsin antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Tissue lysate - other (Synovial fluid)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Other - Agarose amine-reactive support
    Specification
    Synovial fluid
    Read More

    Dr. Tania Colasanti

    Verified customer

    投稿 Jun 03 2016

    Western blot abreview for Anti-alpha 1 Antitrypsin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - other (Synovial fluid)
    Gel Running Conditions
    Reduced Denaturing (7% gel SDS PAGE)
    Loading amount
    2 µg
    Specification
    Synovial fluid
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Tania Colasanti

    Verified customer

    投稿 May 23 2016

    ELISA abreview for Anti-alpha 1 Antitrypsin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (Native)
    Specification
    Native
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Type
    Indirect
    Read More

    Dr. Tania Colasanti

    Verified customer

    投稿 May 23 2016

    ELISA abreview for Anti-alpha 1 Antitrypsin antibody

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Rabbit Purified protein (purified protein)
    Specification
    purified protein
    Type
    Sandwich (Capture)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.25% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 12 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-alpha 1 Antitrypsin antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (liver)
    Specification
    liver
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    GTx30972 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 21 2012

    Question

    Hi,



    I have ordered 2 vials of the Rabbit polyclonal to alpha 1 Antitrypsin antibody, which I got delivered yesterday. I had a look at the datasheet that came with it, unfortunately I couldn’t see the protein concentration on it or on the vials. Is it possible for you to tell me what the protein concentration is on this batch – Product no. ab9373, Lot no. GR77733(N/A). Your datasheet does gives the information about using 1ug/ml concentration for an IHC protocol.

    Many Thanks for your help.

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2012

    Answer

    Thank you for your inquiry.

    It is not possible to provide a exact concentration of this antibody.

    ab9373 was purified using Protein A. This means that also other non Antitrypsin specific IgGs will be present.

    The total protein concentration is approximately 4mg/ml and we estimate that the specific Antitrypsinantibody is about 10%- 20%of this.

    I am sorry I could not provide you with an more exact answer to your question and hope that this information will be nevertheless helpful.

    Read More

    Abcam Scientific Support

    Answered on May 17 2012

    Question

    Hi This datasheet says it is purified IgG, what is the concentration Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Apr 30 2003

    Answer

    The concentration is 400 ug/ml.

    Read More

    Abcam Scientific Support

    Answered on May 01 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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