製品名Anti-ALKBH1 antibody [EPR6176] - BSA and Azide free
ALKBH1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6176] to ALKBH1 - BSA and Azide free
アプリケーション適用あり: IHC-P, IP, WBmore details
Synthetic peptide within Human ALKBH1 aa 350 to the C-terminus. The exact sequence is proprietary.
ab248120 is the carrier-free version of ab126596 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 2.80 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
機能Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
配列類似性Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
細胞内局在Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
- Information by UniProt
- ABH antibody
- ABH1 antibody
- alkB antibody
Lanes 1-2 : Anti-ALKBH1 antibody [EPR6176] - BSA and Azide free (ab248120) at 1/1000 dilution
Lanes 3-4 : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126596).
ab126596 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab126596 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab248120 has not yet been referenced specifically in any publications.