Key features and details
- Alkaline Phosphatase Goat polyclonal to GFP
- Suitable for: WB, ELISA, Dot blot
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
製品名Alkaline Phosphatase Anti-GFP antibody
GFP 一次抗体 製品一覧
製品の詳細Alkaline Phosphatase Goat polyclonal to GFP
特異性Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.
アプリケーション適用あり: WB, ELISA, Dot blotmore details
種交差性交差種: Species independent
Fusion protein corresponding to GFP aa 1-246.
Database link: P42212
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファーPreservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, anti-Alkaline Phosphatase and purified and partially purified GFP (Aequorea victoria) serum.
一次抗体 備考Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Our Abpromise guarantee covers the use of ab6661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500.|
|ELISA||1/2000 - 1/8000.
This product has been assayed against 1.0 µg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
関連性Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
- GFP antibody
- Green fluorescent protein antibody
Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution + GFP at 0.05 µg with Blocking buffer
Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.
Lane 1: 100 ng GFP protein
Lane 2: 33.33 ng GFP protein
Lane 3: 11.11 ng GFP protein
Lane 4: 3.70 ng GFP protein
Lane 5: 1.23 ng GFP protein
ab6661 は 1 報の論文で使用されています。
- Oravcová M et al. Fission yeast CSL proteins function as transcription factors. PLoS One 8:e59435 (2013). WB . PubMed: 23555033