Key features and details
- Rabbit polyclonal to Alkaline Phosphatase
- Reacts with: Escherichia coli
- Isotype: IgG
製品名Anti-Alkaline Phosphatase antibody
Alkaline Phosphatase 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Alkaline Phosphatase
特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
種交差性交差種: Escherichia coli
Full length protein (E. coli).
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by a delipidation and defibrination.
配列類似性Belongs to the alkaline phosphatase family.
- Information by UniProt
- Alkaline phosphatase, germ cell antibody
- Alkaline phosphatase, testicular and thymus antibody
- Akp2 antibody
Ab354 staining Alkaline Phosphatase in E.coli recombinant protein Mach1 by indirect ELISA. Samples were blocked with 5%milk for 3 hours at room temperature and incubated with primary antibody (1/15000 in TBST + 5% Milk) for 90 minutes. An HRP-conjugated goat polyclonal to Rabbit IgG (1/30000) was used as secondary antibody.
All lanes : Anti-Alkaline Phosphatase antibody (ab354) at 1/8000 dilution
All lanes : Escherichia coli Recombinant protein (Mach1)
Lysates/proteins at 0.5 µg per lane.
All lanes : An HRP-conjugated anti-rabbit IgG (whole molecule) at 1/16000 dilution
Developed using the ECL technique.
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
ab354 は 4 報の論文で使用されています。
- Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). PubMed: 30043216
- Bross P et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . PubMed: 18400758
- Imperi F et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). PubMed: 18757814
- Downer R et al. The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. J Biol Chem 277:243-50 (2002). PubMed: 11684686