Alexa Fluor® 700 Anti-CD34 抗体 [4H11[APG]] (ab270669)

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Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
  • Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
  • Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)

Key features and details

  • Alexa Fluor® 700 Mouse monoclonal [4H11[APG]] to CD34
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 700
  • Isotype: IgG1

製品の概要

  • 製品名

    Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]]
    CD34 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 700 Mouse monoclonal [4H11[APG]] to CD34

  • 由来種

    Mouse

  • 標識

    Alexa Fluor® 700

  • 特異性

    This antibody reacts with extracellular class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • 免疫原

    Tissue, cells or virus corresponding to CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

  • ポジティブ･コントロール

    • Flow Cyt: Human peripheral whole blood.

  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
アプリケーション Abreviews 特記事項
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.
特記事項
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

ターゲット情報

  • 機能

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

  • 組織特異性

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

  • 配列類似性

    Belongs to the CD34 family.

  • 発生段階

    On early hematopoietic progenitor cells.

  • 翻訳後修飾

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    画像

    • Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
      Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)

      Flow cytometry analysis of human peripheral whole blood labeling CD34 using ab270669. Surface staining.

    • Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
      Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)

      Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red).

      Control: Lymphocytes (Black). 

    • Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
      Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)

      Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red). Surface staining.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270669 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270669 は論文での使用が確認できていません。

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