Alexa Fluor® 555 Anti-LAG-3 抗体 [EPR20261] (ab280858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR20261] to LAG-3
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 555 Anti-LAG-3 antibody [EPR20261]
LAG-3 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 555 Rabbit monoclonal [EPR20261] to LAG-3 -
由来種
Rabbit -
標識
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- IHC-P: Human Hodgkin's lymphoma tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20261 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-LAG-3 antibody [EPR20261] (ab209236)
- PE Anti-LAG-3 antibody [EPR20261] (ab225484)
- Alexa Fluor® 488 Anti-LAG-3 antibody [EPR20261] (ab225485)
- Alexa Fluor® 647 Anti-LAG-3 antibody [EPR20261] (ab225486)
- Anti-LAG-3 antibody [EPR20261] - BSA and Azide free (ab227579)
- Anti-LAG-3 antibody [EPR20261] - Low endotoxin, Azide free (ab282638)
-
Dyes/Markers
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab280858の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
特記事項 |
---|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
組織特異性
On cell surface of activated NK and T-lymphocytes. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
-
別名
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
画像
-
Immunofluorescence staining of LAG-3 in a section of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma*.
Performed on a Leica BOND®. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab280858 at 1/50 dilution (shown in green) and counterstained using ab190573, Rabbit anti-Tubulin (A647)(1/250) at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab280858 は論文での使用が確認できていません。