Alexa Fluor® 488 Anti-TOMM20 抗体 [EPR15581-39] - Mitochondrial Marker (ab205486)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
TOMM20 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- ICC/IF: HepG2 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15581-39 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205487)
- HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
- Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] (ab214409)
- Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (ab309166)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab309167)
- Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) (ab314475)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab205486の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/1000.
|
特記事項 |
---|
ICC/IF
1/1000. |
ターゲット情報
-
機能
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. -
配列類似性
Belongs to the Tom20 family. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 9804 Human
- Entrez Gene: 67952 Mouse
- Entrez Gene: 266601 Rat
- Omim: 601848 Human
- SwissProt: Q15388 Human
- SwissProt: Q9DCC8 Mouse
- SwissProt: Q62760 Rat
- Unigene: 533192 Human
see all -
別名
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
see all
画像
-
ab205486 staining TOMM20 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205486 at a 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab205486 は 8 報の論文で使用されています。
- Kamradt ML et al. NIK promotes metabolic adaptation of glioblastoma cells to bioenergetic stress. Cell Death Dis 12:271 (2021). PubMed: 33723235
- Jiang L et al. Dual mRNA therapy restores metabolic function in long-term studies in mice with propionic acidemia. Nat Commun 11:5339 (2020). PubMed: 33087718
- Subramani J et al. Thioredoxin protects mitochondrial structure, function and biogenesis in myocardial ischemia-reperfusion via redox-dependent activation of AKT-CREB- PGC1a pathway in aged mice. Aging (Albany NY) 12:19809-19827 (2020). PubMed: 33049718
- Loeffler D et al. Asymmetric lysosome inheritance predicts activation of haematopoietic stem cells. Nature N/A:N/A (2019). PubMed: 31485073
- Park HE et al. Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio. Adv Sci (Weinh) 6:1901673 (2019). PubMed: 31763149
- Holmila RJ et al. Mitochondria-targeted Probes for Imaging Protein Sulfenylation. Sci Rep 8:6635 (2018). PubMed: 29703899
- Ludtmann MHR et al. a-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease. Nat Commun 9:2293 (2018). ICC/IF . PubMed: 29895861
- Ryan TE et al. Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants. JCI Insight 3:N/A (2018). PubMed: 30385731