Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta 抗体 [Y92] (ab196376)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: NIH3T3 cells. WB: N-GST tagged Human PDGF Receptor beta (aa557 to 1106) recombinant protein, N-GST tagged Human PDGF Receptor alpha (aa550 to 1089) recombinant protein.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y92 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- HRP Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab195515)
- Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
- Alexa Fluor® 405 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206873)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - Low endotoxin, Azide free (ab215978)
- PE Anti-PDGFR alpha + PDGFR beta antibody [Y92] (C-terminal) (ab307638)
- APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab314942)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab32570)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/50.
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特記事項 |
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ICC/IF
1/50. |
ターゲット情報
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細胞内局在
PDGFR alpha: Membrane. PDGFR beta: Membrane. -
参照データベース
- Entrez Gene: 18595 Mouse
- Entrez Gene: 18596 Mouse
- SwissProt: P05622 Mouse
- SwissProt: P26618 Mouse
- Unigene: 221403 Mouse
- Unigene: 4146 Mouse
画像
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ab196376 staining PDGFR alpha + beta in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196376 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab196376 は論文での使用が確認できていません。