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リコンビナントRabMAb

Alexa Fluor® 488 Anti-Fatty Acid Synthase 抗体 [EPR7466] (ab197485)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
  • Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR7466] to Fatty Acid Synthase
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Carrier Free HRP Unconjugated

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466]
    Fatty Acid Synthase 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR7466] to Fatty Acid Synthase

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: A549 cells. Flow Cyt (intra): A549 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab197485の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.

This product gave a positive signal in A549 cells fixed with 100% methanol (5 min).
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/100.

This product gave a positive signal in A549 cells fixed with 100% methanol (5 min).
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

  • 組織特異性

    Ubiquitous. Prominent expression in brain, lung, and liver.

  • 配列類似性

    Contains 1 acyl carrier domain.

  • 細胞内局在

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)

    Overlay histogram showing A549 cells stained with ab197485 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197485, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in A549 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)

    ab197485 staining Fatty Acid Synthase in A549 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197485 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)
    Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7466] (ab197485)

参考文献 (1)

ab197485 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab197485 は 1 報の論文で使用されています。

  • Lee JI  et al. Functional recovery with histomorphometric analysis of nerves and muscles after combination treatment with erythropoietin and dexamethasone in acute peripheral nerve injury. PLoS One 15:e0238208 (2020). PubMed: 32881928

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