Alexa Fluor® 488 Anti-Choline Acetyltransferase 抗体 [EPR13024(B)] (ab192465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Rat, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR13024(B)]
Choline Acetyltransferase 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IF, IHC-Frmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-Fr: rat frozen dentate gyrus tissue section
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13024(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab192466)
- PE Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab224000)
- APC Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab224001)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225262)
- Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225263)
- Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab302805)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
|
|
IHC-Fr |
1/100.
This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min). |
特記事項 |
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ICC/IF
1/100. |
IHC-Fr
1/100. This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min). |
ターゲット情報
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機能
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
関連疾患
Myasthenic syndrome, congenital, 6, presynaptic -
配列類似性
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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参照データベース
- Entrez Gene: 1103 Human
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- Omim: 118490 Human
- SwissProt: P28329 Human
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 302002 Human
see all -
別名
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
画像
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IHC image of Choline Acetyltransferase staining in a section of frozen normal rat dentate gyrus.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab192465 at 1/100 (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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ab192465 staining Choline Acetyltransferase in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192465 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab192465 は 3 報の論文で使用されています。
- Gärtner F et al. Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex. Front Immunol 12:745132 (2021). PubMed: 34867969
- Sen MK et al. Behavioural and histological changes in cuprizone-fed mice. Brain Behav Immun 87:508-523 (2020). PubMed: 32014578
- Lee JH et al. Dopaminergic Regulation of Nucleus Accumbens Cholinergic Interneurons Demarcates Susceptibility to Cocaine Addiction. Biol Psychiatry 88:746-757 (2020). PubMed: 32622465