リコンビナントRabMAb

Alexa Fluor® 488 Anti-CD39 抗体 [EPR22507-48] (ab270916)

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Immunocytochemistry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
  • Flow Cytometry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
  • Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR22507-48] to CD39
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

製品の概要

  • 製品名

    Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48]
    CD39 一次抗体 製品一覧

  • 製品の詳細

    Alexa Fluor® 488 Rabbit monoclonal [EPR22507-48] to CD39

  • 由来種

    Rabbit

  • 標識

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt: C57 BL/6 mouse splenocytes. ICC/IF: Mouse splenocytes.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR22507-48

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
ICC
Mouse
アプリケーション Abreviews 特記事項
Flow Cyt
1/2500.
ICC
1/500.

This product gave a positive signal in Mouse Splenocytes fixed with 4% formaldehyde (10 min).
特記事項
Flow Cyt
1/2500.
ICC
1/500.

This product gave a positive signal in Mouse Splenocytes fixed with 4% formaldehyde (10 min).

ターゲット情報

  • 機能

    In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.

  • 組織特異性

    Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.

  • 配列類似性

    Belongs to the GDA1/CD39 NTPase family.

  • 翻訳後修飾

    The N-terminus is blocked.
    Palmitoylated in the N-terminal part.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      There are 3 isoforms produced by alternative splicing.

    • 別名

      • ATPDase antibody
      • CD 39 antibody
      • CD39 antibody
      • CD39 antigen antibody
      • DKFZp686D194 antibody
      • DKFZp686I093 antibody
      • Ecto apyrase antibody
      • Ecto ATP diphosphohydrolase antibody
      • Ecto-apyrase antibody
      • Ecto-ATP diphosphohydrolase 1 antibody
      • Ecto-ATPase 1 antibody
      • Ecto-ATPDase 1 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
      • ENTP1_HUMAN antibody
      • ENTPD 1 antibody
      • ENTPD1 antibody
      • FLJ40921 antibody
      • FLJ40959 antibody
      • Lymphoid cell activation antigen antibody
      • NTPDase 1 antibody
      • NTPDase1 antibody
      • SPG64 antibody
      see all

    画像

    • Immunocytochemistry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
      Immunocytochemistry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)

      Immunofluorescence staining of CD39 in a subset of mouse splenocytes using ab270916. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270916 at 1/500 dilution (shown in green) and ab190573, Rabbit monoclonal to alphaTubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
      Flow Cytometry - Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)

      Flow cytometry staining of C57 BL/6 mouse splenocytes with ab270916 (right) or Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab270916) or Rabbit IgG (monoclonal) Alexa Fluor 488® (ab199091) isotype (1x 106 in 100µl at 0.2µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD45R.

      Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

      Events were gated on live lymphocytes.

    • Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)
      Alexa Fluor® 488 Anti-CD39 antibody [EPR22507-48] (ab270916)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270916 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270916 は論文での使用が確認できていません。

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