Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR23009-76] to CD30-L
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
製品名Alexa Fluor® 488 Anti-CD30-L antibody [EPR23009-76]
CD30-L 一次抗体 製品一覧
製品の詳細Alexa Fluor® 488 Rabbit monoclonal [EPR23009-76] to CD30-L
標識Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested Applications & Species
Application Species Flow CytMouse
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow Cyt: C57 BL/6 mouse splenocytes (+/- treated with ConA (2.5 µg/ml) 3 days).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS
Concentration information loading...
精製度Protein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
機能Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
配列類似性Belongs to the tumor necrosis factor family.
- Information by UniProt
- CD153 antibody
- CD153 antigen antibody
- CD30 antigen ligand antibody
Flow cytometry staining of C57 BL/6 mouse splenocytes (top) or C57 BL/6 mouse splenocytes treated with ConA (2.5 µg/mL, 3 days) (bottom), with ab270182 (right) or Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) isotype (left). C57 BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab270182) or Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) isotype (1x 106 in 100µL at 1 µg/mL (1/500)) for 30 min on ice. The cells were simultaneously stained with CD4.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live lymphocytes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.