Anti-ALDH5A1/SSADH 抗体 [EPR7794] - BSA and Azide free (ab248262)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7794] to ALDH5A1/SSADH - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALDH5A1/SSADH antibody [EPR7794] - BSA and Azide free
ALDH5A1/SSADH 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7794] to ALDH5A1/SSADH - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248262 is the carrier-free version of ab129017.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.16 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7794 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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機能
Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). -
組織特異性
Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta. -
パスウェイ
Amino-acid degradation; 4-aminobutanoate degradation. -
関連疾患
Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. -
配列類似性
Belongs to the aldehyde dehydrogenase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 7915 Human
- Entrez Gene: 214579 Mouse
- Entrez Gene: 291133 Rat
- Omim: 610045 Human
- SwissProt: P51649 Human
- SwissProt: Q8BWF0 Mouse
- SwissProt: P51650 Rat
- Unigene: 371723 Human
see all -
別名
- Aldedehyde dehydrogenase 5 family antibody
- Aldehyde dehydrogenase 5 family member A1 antibody
- Aldehyde dehydrogenase 5A1 antibody
see all
画像
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Anti-ALDH5A1/SSADH antibody [EPR7794] (ab129017) at 1/10000 dilution + Fetal liver tissue lysate at 10 µg
Predicted band size: 57 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab129017, the same antibody clone in a different buffer formulation.
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This data was developed using ab129017, the same antibody clone in a different buffer formulation.ab129017, at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Human liver tissue by immunohistochemistry Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab129017, the same antibody clone in a different buffer formulation.ab129017, at 1/100 dilution staining ALDH5A1/SSADH in formalin fixed paraffin embedded Mouse kidney tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab129017, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248262 は論文での使用が確認できていません。