Anti-ALDH1L1 + ALDH1L2 抗体 [EPR12743(B)] - BSA and Azide free (ab233016)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12743(B)] to ALDH1L1+ALDH1L2 - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - BSA and Azide free
ALDH1L1+ALDH1L2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12743(B)] to ALDH1L1+ALDH1L2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human kidney tissue. IHC-Fr: Mouse and rat liver tissue.
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特記事項
ab233016 is the carrier-free version of ab177463.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12743(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
- Alexa Fluor® 647 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] (ab311145)
- Alexa Fluor® 594 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] (ab311786)
- Alexa Fluor® 568 Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab313067)
- Alexa Fluor® 555 Anti-ALDH1L1 antibody [EPR12743(B)] (ab314254)
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233016の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 99 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 99 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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細胞内局在
ALDH1L1: Cytoplasm. -
参照データベース
- Entrez Gene: 10840 Human
- Entrez Gene: 160428 Human
- Entrez Gene: 107747 Mouse
- Entrez Gene: 216188 Mouse
- Entrez Gene: 299699 Rat
- Entrez Gene: 64392 Rat
- Omim: 600249 Human
- Omim: 613584 Human
see all
画像
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All lanes : Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : His-tagged mouse ALDH1L1 fragment recombinant protein (aa162-366)
Lane 2 : His-tagged mouse ALDH1L2 fragment recombinant protein (aa184-387)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
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Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on mouse liver.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
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Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on rat liver.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177463).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab233016 は論文での使用が確認できていません。