製品名Anti-ALDH1L1 antibody - Astrocyte Marker
ALDH1L1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
アプリケーション適用あり: WB, IHC-P, ICC/IF, IHC-Fr, ICCmore details
種交差性交差種: Mouse, Rat
- WB: Mouse liver; mouse brain and rat brain lysates. IHC-P: Mouse brain tissue. IHC-Fr: Mouse brain-astrocytes tissue.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab87117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml. PubMed: 24501362|
|ICC||Use at an assay dependent concentration.|
組織特異性Highly expressed in liver, pancreas and kidney.
配列類似性In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Contains 1 acyl carrier domain.
- Information by UniProt
- 10 formyltetrahydrofolate dehydrogenase antibody
- 10 FTHFDH antibody
- 10-formyltetrahydrofolate dehydrogenase antibody
PF-PBS post-fixed lamina frozen sections treated with rNAION for 7 days to induce ischaemia, were analyzed for ALDH1L1 (Red)(ab87117) at 1:1000 dilution. Antigen recovery using citrate buffer was performed. There is extensive dendrimer signal overlap with the astrocyte cytoplasm (arrows).
All lanes : Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
IHC-P image of ALDH1L1 staining on Mouse brain tissue section using ab87117. The sections were subjected to heat-mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 minutes at 21°C and then incubated with ab87117 (1/1000 dilution) for 2 hours at 21°C.
ab87117 staining ALDH1L1 in mouse brain-astrocytes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Paraffin-embedded rat brain tissue stained for ALDH1L1 using ab87117 at 1/1000 dilution in immunohistochemical analysis.
Astrocytes are clearly labeled: red for those attaching to the pia mater, blue for those in the grey matter and green for astrocytes that are intimately associated with blood vessels.
This product has been referenced in:
- Wu R et al. A novel m6A reader Prrc2a controls oligodendroglial specification and myelination. Cell Res 29:23-41 (2019). Read more (PubMed: 30514900) »
- Ignatenko O et al. Loss of mtDNA activates astrocytes and leads to spongiotic encephalopathy. Nat Commun 9:70 (2018). Read more (PubMed: 29302033) »