製品の概要

  • 製品名
    Anti-ALDH1L1 antibody - Astrocyte Marker
    ALDH1L1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide within Mouse ALDH1L1 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q8R0Y6
    (Peptide available as ab103935)

  • ポジティブ・コントロール
    • WB: Mouse liver; mouse brain and rat brain lysates. IHC-P: Mouse brain tissue. IHC-Fr: Mouse brain-astrocytes tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml. PubMed: 24501362
IHC-Fr 1/1000.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性
    Highly expressed in liver, pancreas and kidney.
  • 配列類似性
    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

画像

  • PF-PBS post-fixed lamina frozen sections treated with rNAION for 7 days to induce ischaemia, were analyzed for ALDH1L1 (Red)(ab87117) at 1:1000 dilution. Antigen recovery using citrate buffer was performed. There is extensive dendrimer signal overlap with the astrocyte cytoplasm (arrows).

  • All lanes : Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • IHC-P image of ALDH1L1 staining on Mouse brain tissue section using ab87117. The sections were subjected to heat-mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 minutes at 21°C and then incubated with ab87117 (1/1000 dilution) for 2 hours at 21°C.

    See Abreview

  • ab87117 staining ALDH1L1 in mouse brain-astrocytes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Paraffin-embedded rat brain tissue stained for ALDH1L1 using ab87117 at 1/1000 dilution in immunohistochemical analysis.

    Astrocytes are clearly labeled: red for those attaching to the pia mater, blue for those in the grey matter and green for astrocytes that are intimately associated with blood vessels.

    See Abreview

参考文献

This product has been referenced in:
See all 19 Publications for this product

レビューと Q&A

1-10 of 10 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jun 08 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Mouse Tissue sections (brain-astrocytes)
Specification
brain-astrocytes
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 05 2014

Answer

I am very pleased to hear you would like to accept our offer and test ab87117 in human samples.

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for human samples and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us.

We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for your reply.

ab87117 is suitable for paraffin-embedded sections but it hasnt been tested on human samples yet. Therefore, I can offer a discount off a future purchase if you buy ab87117 now, test it in human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab87117 in human. I will then send a discount code. This code must be issued before purchasing ab87117 so please wait for my reply before ordering.

2. Purchase ab87117 either by phone, fax, or online (www.abcam.com).

3. Test it in human.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab87117 turns out to be unsuitable for human, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (mouse cortex)
Specification
mouse cortex
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton x100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 29 2012

Answer

Thank you for providing that information. I have now generated the discount code for your customer:
Discount code: xxxxxx
Expiration date: 11th July 2012
In order to activate the code, please advise your customer to submit an Abreview for ICC with ab87117, quoting the discount code in the "additional comments" section of the form. Once this has been completed the free primary antibody can be claimed within the expiration date given above. More information on this can be found from the following link:
www.abcam.com/collaborationdiscount
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for contacting us.

Yes, that information would be very valuable for us and our customers. Ab87117 has previously been used by one of our customers to perform ICC with rat cultured cells. Although the results were not as hoped, the protocol could possible have been improved on to obtain better staining. I would suggest your customer use 1/200 dilution initially, incubating overnight at 4 degrees C. If your customer has problems obtaining good staining, please get in contact with us prior to submitting the Abreview to see if we can offer any suggestions to improve the results.

If your customer would like to participate in the testing discount scheme please could you provide me with the following details:
1. Their name
2. Their email address
3. Their institution

Once I have received this information I will issues a discount code for your customer. Alternatively, we have a mouse monoclonal against ALDH1L1 (ab56777) which has been tested with mouse and ICC and would therefore be guaranteed for the application your customer wishes to perform.

More information on the testing discount scheme can be found from the following link:

www.abcam.com/collaborationdiscount

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Astrocytes)
Permeabilization
Yes - Triton-X100
Specification
Astrocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde

Rehana Leak

Verified customer

投稿 Jul 01 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain cortex)
Specification
Brain cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

投稿 May 03 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

投稿 Feb 24 2011

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