Anti-ALDH1L1 抗体 (ab235197)
Key features and details
- Goat polyclonal to ALDH1L1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALDH1L1 antibody
ALDH1L1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ALDH1L1 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant mouse ALDH1L1 protein; Mouse liver tissue lysate. ICC/IF: NIH/3T3 cells. IHC-P: Mouse kidney and liver tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab235197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 99 kDa.
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ICC/IF |
Use a concentration of 15 µg/ml.
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IHC-P |
1/200.
HIER using Citrate Buffer for 20 minutes. |
特記事項 |
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WB
1/1000. Predicted molecular weight: 99 kDa. |
ICC/IF
Use a concentration of 15 µg/ml. |
IHC-P
1/200. HIER using Citrate Buffer for 20 minutes. |
ターゲット情報
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組織特異性
Highly expressed in liver, pancreas and kidney. -
配列類似性
In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Contains 1 acyl carrier domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10840 Human
- Entrez Gene: 107747 Mouse
- Entrez Gene: 64392 Rat
- Omim: 600249 Human
- SwissProt: O75891 Human
- SwissProt: Q8R0Y6 Mouse
- SwissProt: Q5RFM9 Orangutan
- SwissProt: P28037 Rat
see all -
別名
- 10 formyltetrahydrofolate dehydrogenase antibody
- 10 FTHFDH antibody
- 10-formyltetrahydrofolate dehydrogenase antibody
see all
画像
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All lanes : Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution
Lane 1 : Opal pre-stained ladder
Lane 2 : Recombinant mouse ALDH1L1 protein
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5 : Mouse liver (normal tissue) lysate
Secondary
Lanes 1-4 : F(ab)'2 Dk-a-Gt IgG [H&L] at 1/40000 dilution
Lane 5 : Goat polyclonal to ALDH1L1 (ab235197) at 1/40000 dilution (F(ab)'2 Dk-a-Gt IgG [H&L])
Predicted band size: 99 kDa
Observed band size: 98.7 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).
Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.
A) DAPI
B) ab235197 + secondary.
C) Merged image
D) Secondary only control.Expected localization: Cytoplasm.
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100% methanol-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).
Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.
A) DAPI
B) ab235197 + secondary.
C) Merged image
D) Secondary only control.Expected localization: Cytoplasm.
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Immunohistochemical analysis of mouse kidney tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.
Pathologist analysis: ALDH1L1 showed selective staining of renal tubular epithelial cells in mouse kidney. The specificity of the staining is cytoplasmic and membranous in tubules not in glomeruli which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1:200.
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Immunohistochemical analysis of mouse liver tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.
Pathologist analysis: ALDH1L1 showed selective cytoplasmic staining in mouse liver of hepatocytes. The specificity of the staining is weak in bile duct cells and strong membranous and cytoplasmic in hepatocytes which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1:200.
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All lanes : Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : ALDH1L1 HEK293T overexpressed lysate
Secondary
All lanes : 605-703-125 GOAT IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed at 1/40000 dilution
Predicted band size: 99 kDa
Exposure time: 1 secondALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).
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Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
Goat polyclonal to ALDH1L1 (ab235197) at 1/40000 dilution (605-703-125 GOAT IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed)
Predicted band size: 99 kDa
Observed band size: 80, 34 kDa why is the actual band size different from the predicted?
Exposure time: 35 secondsALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab235197 は論文での使用が確認できていません。