Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (MDBK)
Specification
MDBK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Interestingly, ALDH1A2 staining under the above mentioned experimental conditions shows both cytoplasmic and nuclear localization. Touma et al. (Biochem Pharmacol 2009, 78:1127-1138) have also shown previously that ALDH1A2 is localized to both cytoplasm and nucleus in murine prostate paraffin sections.
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投稿 Nov 08 2012