Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase
Mouse, Rat, Human
Synthetic peptide within Human Alcohol Dehydrogenase aa 1-100. The exact sequence is proprietary.
WB: lysate from HepG2 cells, Human fetal, mouse or rat liver;
IHC-P: Human liver tissue;
ICC/IF: HepG2 cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Is unsuitable for Flow Cyt or IP.
Belongs to the zinc-containing alcohol dehydrogenase family.
Information by UniProt
ADH alpha subunit antibody
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) This image is courtesy of an Abreview submitted by Armen Petrosyan
ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).
Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilution Lane 1 : HepG2 cell lysate Lane 2 : Mouse liver lysate Lane 3 : Human fetal liver lysate Lane 4 : Rat liver lysate Lysates/proteins at 10 µg per lane. Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203)
ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"