Albumin Pig ELISA Kit (ab108795)
Key features and details
- Sensitivity: 3 µg/ml
- Range: 6.25 µg/ml - 50 µg/ml
- Sample type: Cell culture extracts, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Pig
製品の概要
-
製品名
Albumin Pig ELISA Kit
Albumin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Serum, Plasma, Cell culture extracts, Cell Lysate -
アッセイタイプ
Competitive -
検出感度
= 3 µg/ml -
検出範囲
6.25 µg/ml - 50 µg/ml -
添加回収試験
96 %
-
全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Pig -
製品の概要
Abcam’s Albumin Pig in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 3X Biotinylated Pig Albumin (Lyophilized) 1 vial Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
-
機能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
組織特異性
Plasma. -
関連疾患
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all
画像
-
Typical Standard Curve
Representative Standard Curve using ab108795
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab108795 は 1 報の論文で使用されています。
- Montanari E et al. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. J Immunol Res 2018:1078547 (2018). PubMed: 29577046