リコンビナントRabMAb

Anti-Albumin 抗体 [EPR20195] - BSA and Azide free (ab271979)

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Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
  • Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
  • Flow Cytometry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
  • Immunoprecipitation - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
  • Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20195] to Albumin - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Albumin antibody [EPR20195] - BSA and Azide free
    Albumin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20195] to Albumin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WB, Flow Cyt, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
    Database link: P02768

  • ポジティブ･コントロール

    • WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. CC/IF: HepG2 cells.Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.

  • 特記事項

    ab271979 is the carrier-free version of ab207327. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab271979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
Flow Cyt Use at an assay dependent concentration.

Permeabilization is required.

 
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • 組織特異性

    Plasma.

  • 関連疾患

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

  • 配列類似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 翻訳後修飾

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    There are 2 isoforms produced by alternative splicing.

  • 別名

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • Growth inhibiting protein 20 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

画像

  • Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
    Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

    Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture.

    Blue: DAPI, Green: CD68, Yellow: Albumin, Red: Vimentin

    Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
  • Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
    Immunocytochemistry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    Negative control: HeLa (PMID: 10476216 and 8314088)).

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
  • Flow Cytometry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
    Flow Cytometry - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

    Flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albumin with ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).

  • Immunoprecipitation - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
    Immunoprecipitation - Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

    Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab207327 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
  • Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
    Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271979 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271979 は論文での使用が確認できていません。

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