Anti-AKT1 (phospho S473) 抗体 (ab8932)
Key features and details
- Rabbit polyclonal to AKT1 (phospho S473)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AKT1 (phospho S473) antibody
AKT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKT1 (phospho S473) -
由来種
Rabbit -
特異性
The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue. -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Cow, Human -
免疫原
Synthetic peptide: CHFPQFSpYSAS conjugated to KLH, corresponding to C terminal amino acids 468-477 of Human AKT.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab8932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/100. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. |
ターゲット情報
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機能
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. -
組織特異性
Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. -
関連疾患
Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1). -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
The AGC-kinase C-terminal mediates interaction with THEM4. -
翻訳後修飾
Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 280991 Cow
- Entrez Gene: 207 Human
- Omim: 164730 Human
- SwissProt: Q01314 Cow
- SwissProt: P31749 Human
- Unigene: 525622 Human
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別名
- AKT 1 antibody
- AKT antibody
- AKT1 antibody
see all
画像
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Western blot - Anti-AKT1 (phospho S473) antibody (ab8932)ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Normal human breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
This image is a magnification of the accompanying breast tumor image.
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Image from Meyer S et al., PLoS One. 2012;7(6):e38222. Epub 2012 Jun 7. Fig S5.; doi:10.1371/journal.pone.0038222; June 7, 2012, PLoS ONE 7(6): e38222.Immunohistochemical analysis of Human prostate cancer and melanoma cell lines, staining AKT1 (phospho S473) with ab8932. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
プロトコール
参考文献 (44)
ab8932 は 44 報の論文で使用されています。
- Dong H et al. Activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast Cancer. Mol Cancer 18:3 (2019). PubMed: 30621694
- Zhang X et al. Tanshinone IIA induces apoptosis of ovarian cancer cells in vitro and in vivo through attenuation of PI3K/AKT/JNK signaling pathways. Oncol Lett 17:1896-1902 (2019). PubMed: 30675253
- Chen F et al. lncRNA PLAC2 activated by H3K27 acetylation promotes cell proliferation and invasion via the activation of Wnt/ß-catenin pathway in oral squamous cell carcinoma. Int J Oncol 54:1183-1194 (2019). PubMed: 30720068
- Man X et al. USP13 functions as a tumor suppressor by blocking the NF-kB-mediated PTEN downregulation in human bladder cancer. J Exp Clin Cancer Res 38:259 (2019). PubMed: 31200745
- Gu Z et al. USP34 regulated human pancreatic cancer cell survival via AKT and PKC pathways. Biol Pharm Bull N/A:N/A (2019). PubMed: 30686807
