Akt (pS473) + total Akt ELISA Kit (ab126433)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Akt (pS473) + total Akt ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell Lysate -
アッセイタイプ
Semi-quantitative -
全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse, Rat, Human -
製品の概要
ab126433 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Akt protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Akt (Ser473) and total Akt in human, mouse and rat cell lysates (help normalize the results of phospho-Akt from different cell lysate being compared). A pan Akt antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Akt present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Akt (Ser473) or anti-pan-Akt is used to detect phosphorylated or total Akt. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Akt (Ser473) or total Akt bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with AKT 1/2/3 pS473 + AKT1 ELISA Kit (ab176657) from our SimpleStep ELISA® range.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 500X HRP-conjugated anti-rabbit IgG 1 x 25µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml Akt Microplate (12 strips x 8 wells) coated with anti-pan Akt antibody 1 unit Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473) 1 vial Detection Antibody Akt: rabbit anti-pan-Akt 1 vial Positive Control: lyophilized NIH3T3 cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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細胞内局在
AKT1/2/3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. AKT1 (total): Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. -
別名
- AKT
- AKT 1
- AKT1
see all -
参照データベース
- Entrez Gene: 10000 Human
- Entrez Gene: 10000 Human
- Entrez Gene: 1385 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 11651 Mouse
see all
画像
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MCF7 cells were stimulated for five minutes with 1 ug x mL-1 of insulin (ab123768). Cell lysates were assessed for total Akt and Akt(pS473), shown as OD (450 nm) after background signal was subtracted (duplicates +/- SD).
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The NIH3T3 cells were treated with recombinant human PDGF for 10 minutes to induce phosphorylation of Akt. Serial dilutions of lysates were analyzed in this ELISA.
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NIH3T3 cells were treated or untreated with recombinant human PDGF for 10 min. Cell lysates were analyzed by Western Blot.
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NIH3T3 cells were treated or untreated with recombinant human PDGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
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A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab126433 は 14 報の論文で使用されています。
- Xu SH et al. Therapeutic Effect of Catgut Implantation at Acupoint in a Mouse Model of Hepatocellular Carcinoma by Suppressing Immune Escape. Evid Based Complement Alternat Med 2022:5572869 (2022). PubMed: 35178106
- Ren SW et al. Exosomes derived from human umbilical cord mesenchymal stem cells promote osteogenesis through the AKT signaling pathway in postmenopausal osteoporosis. Aging (Albany NY) 14:10125-10136 (2022). PubMed: 36575048
- Ying W et al. MiR-690, an exosomal-derived miRNA from M2-polarized macrophages, improves insulin sensitivity in obese mice. Cell Metab 33:781-790.e5 (2021). PubMed: 33450179
- Liang L et al. Osthole suppresses the proliferation and induces apoptosis via inhibiting the PI3K/AKT signaling pathway of endometrial cancer JEC cells. Exp Ther Med 22:1171 (2021). PubMed: 34504616
- Abd El-Fattah EE et al. AKT-AMPKα-mTOR-dependent HIF-1α Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma. Front Pharmacol 12:720173 (2021). PubMed: 35095479