Anti-AKR1B10 抗体 (ab96417)
Key features and details
- Rabbit polyclonal to AKR1B10
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-AKR1B10 antibody
AKR1B10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKR1B10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human AKR1B10 aa 1-286.
Database link: O60218 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/1000.
|
特記事項 |
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WB
1/500 - 1/10000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
組織特異性
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
配列類似性
Belongs to the aldo/keto reductase family. -
細胞内局在
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 57016 Human
- Entrez Gene: 67861 Mouse
- Entrez Gene: 296972 Rat
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
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別名
- AK1BA_HUMAN antibody
- AKR1B10 antibody
- AKR1B11 antibody
see all
画像
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Anti-AKR1B10 antibody (ab96417) at 1/5000 dilution + Mouse colon lysate at 50 µg
Predicted band size: 36 kDa -
All lanes : Anti-AKR1B10 antibody (ab96417) at 1/2500 dilution
Lane 1 : Jurkat cells
Lane 2 : Raji cells
Lane 3 : NCI-H929 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa -
Anti-AKR1B10 antibody (ab96417) at 1/50000 dilution + Rat colon lysate at 50 µg
Predicted band size: 36 kDa -
All lanes : Anti-AKR1B10 antibody (ab96417) at 1/1000 dilution
Lane 1 : MOLT-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
ab96417, at 1/200 dilution, staining AKR1B10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab96417 は 8 報の論文で使用されています。
- Hojnik M et al. AKR1B1 as a Prognostic Biomarker of High-Grade Serous Ovarian Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35159076
- Chabert C et al. AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome. Int J Mol Sci 23:N/A (2022). PubMed: 35163833
- Liu C et al. AKR1B10 accelerates the production of proinflammatory cytokines via the NF-κB signaling pathway in colon cancer. J Mol Histol 53:781-791 (2022). PubMed: 35920984
- Tatsuta T et al. Transcriptomic alterations in malignant pleural mesothelioma cells in response to long-term treatment with bullfrog sialic acid-binding lectin. Mol Med Rep 23:N/A (2021). PubMed: 33880588
- Hojnik M et al. AKR1B1 and AKR1B10 as Prognostic Biomarkers of Endometrioid Endometrial Carcinomas. Cancers (Basel) 13:N/A (2021). PubMed: 34298614
- Ghosh A et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . PubMed: 29481290
- Huppke P et al. Activating de novo mutations in NFE2L2 encoding NRF2 cause a multisystem disorder. Nat Commun 8:818 (2017). WB ; Human . PubMed: 29018201
- Hevir N et al. Decreased levels of AKR1B1 and AKR1B10 in cancerous endometrium compared to adjacent non-cancerous tissue. Chem Biol Interact : (2012). WB, IHC-P ; Human . PubMed: 23146748