Anti-AKAP 95 抗体 (ab72196)
Key features and details
- Rabbit polyclonal to AKAP 95
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-AKAP 95 antibody
AKAP 95 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AKAP 95 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to Human AKAP 95 aa 642-692 (C terminal).
Database link: NP_005849.1 -
ポジティブ・コントロール
- HeLa and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 86 kDa (predicted molecular weight: 76 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). -
組織特異性
Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the AKAP95 family.
Contains 2 C2H2 AKAP95-type zinc fingers. -
細胞内局在
Nucleus matrix. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 10270 Human
- Entrez Gene: 56399 Mouse
- Omim: 604692 Human
- SwissProt: O43823 Human
- SwissProt: Q9DBR0 Mouse
- Unigene: 594496 Human
- Unigene: 328945 Mouse
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別名
- A kinase (PRKA) anchor protein 8 antibody
- A kinase anchor protein 8 antibody
- A kinase anchor protein 95 antibody
see all
画像
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All lanes : Anti-AKAP 95 antibody (ab72196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP 95 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
Lane 2 represents a Control IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72196 は 2 報の論文で使用されています。
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Liu W et al. Roles of Cx43 and AKAP95 in ovarian cancer tissues in G1/S phase. Int J Clin Exp Pathol 8:14315-24 (2015). Human . PubMed: 26823747