Anti-AIF 抗体 (ab2086)

Submit a review Q&A (1)References (1)
Western blot - Anti-AIF antibody (ab2086)
  • Immunocytochemistry - Anti-AIF antibody (ab2086)
  • Western blot - Anti-AIF antibody (ab2086)

Key features and details

  • Rabbit polyclonal to AIF
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-AIF antibody
    AIF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to AIF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide:

    KDGEQHEDLNEVAK

    , corresponding to amino acids 593 - 606 of Human AIF.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: CaCo-2, Daudi, HeLa, K562, NIH3T3 and YB2/0 cell lysate. ICC: K562 cells

  • 特記事項

    Apoptosis Inducing Factor.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能

    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

  • 関連疾患

    Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.

  • 配列類似性

    Belongs to the FAD-dependent oxidoreductase family.

  • 翻訳後修飾

    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.

  • 細胞内局在

    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AIFM1 antibody
    • AIFM1_HUMAN antibody
    • Apoptosis inducing factor 1, mitochondrial antibody
    • Apoptosis inducing factor antibody
    • Apoptosis inducing factor, mitochondrion associated, 1 antibody
    • Apoptosis-inducing factor 1 antibody
    • CMTX4 antibody
    • COWCK antibody
    • COXPD6 antibody
    • Harlequin antibody
    • Hq antibody
    • mAIF antibody
    • MGC111425 antibody
    • MGC5706 antibody
    • mitochondrial antibody
    • Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
    • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
    • PDCD 8 antibody
    • PDCD8 antibody
    • Programmed cell death 8 (apoptosis inducing factor) antibody
    • Programmed cell death 8 antibody
    • Programmed cell death 8 isoform 1 antibody
    • Programmed cell death 8 isoform 2 antibody
    • Programmed cell death 8 isoform 3 antibody
    • Programmed cell death protein 8 antibody
    • Programmed cell death protein 8 mitochondrial antibody
    • Programmed cell death protein 8 mitochondrial precursor antibody
    • Programmed cell death protein 8 mitochondrial precursor antibody
    • Striatal apoptosis inducing factor antibody
    see all

画像

  • Western blot - Anti-AIF antibody (ab2086)
    Western blot - Anti-AIF antibody (ab2086)
    All lanes : Anti-AIF antibody (ab2086) at 2 µg/ml

    Lane 1 : CACO-2 cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 67 kDa

  • Immunocytochemistry - Anti-AIF antibody (ab2086)
    Immunocytochemistry - Anti-AIF antibody (ab2086)

    Immunocytochemical analysis of K562 cells with ab2086 at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.

  • Western blot - Anti-AIF antibody (ab2086)
    Western blot - Anti-AIF antibody (ab2086)
    All lanes : Anti-AIF antibody (ab2086) at 2 µg/ml

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : YB2/0 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 67 kDa

参考文献 (1)

ab2086 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2086 は 1 報の論文で使用されています。

  • Wall J  et al. Modeling Cardiac Dysfunction Following Traumatic Hemorrhage Injury: Impact on Myocardial Integrity. Front Immunol 10:2774 (2019). PubMed: 31866998

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Do any of your three antibodies to AIF cross react with chicken?

Read More
Answer

We havn't tested the chicken reactivity for any one. However, the immunogenic peptide sequences of the 2267 (IN) and the 2301 (CT) are highly conserved (identical) in human, mouse and rat. They are likely to be broad species response. If you would like to give us a feed back infomation, we can sell you these two for one price.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com