Key features and details
- Rabbit polyclonal to AICDA - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
製品名Anti-AICDA antibody - ChIP Grade
AICDA 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to AICDA - ChIP Grade
アプリケーション適用あり: WB, ChIPmore details
Synthetic peptide corresponding to Human AICDA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab5197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).|
|ChIP||Use at an assay dependent concentration.|
機能RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.
組織特異性Strongly expressed in lymph nodes and tonsils.
関連疾患Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers.
配列類似性Belongs to the cytidine and deoxycytidylate deaminase family.
- Information by UniProt
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
ab5197 は 2 報の論文で使用されています。
- Dayal S et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . PubMed: 21949728
- Duquette ML et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). PubMed: 15940261