Application
Western blot
Sample
Rat Tissue lysate - whole (dorsal root ganglia explants)
Loading amount
50 µg
Specification
dorsal root ganglia explants
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Amersham ECL Peroxidase labelled anti-rabbit antib
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 97 kDa
Additional data
Additional Notes
ab3593 detects a clear band at ~ 97 kDa. Some non-specific background is seen below this.
Abcam response
Thank you for your review of ab3593. Your image shows a clear band at the expected size for Argonaute 3 / eIF2C 3. You may find that using more frequent, shorter washes will reduce the amount of background staining you see.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 28 2006