Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 37°C · Diluent: PBS plus casein
Secondary antibody
Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lynn Dong
Verified customer
投稿 Feb 28 2012