Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Autoradiogram Exposure Time: 16
Additional data
Additional Notes
UV crosslinking appears to be the limiting step in the iCLIP process, further optimisation of UV crosslinking may be needed before the effieciency of immunoprecipitation by this antibody can be assessed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 21 2012