Application
Western blot
Sample
Human Cell lysate - whole cell (human umbilical vein endothelial cells)
Loading amount
50 µg
Specification
human umbilical vein endothelial cells
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated anti-Ribbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
45 second(s)
Bands
Specific: about 95 kDa
Positive control
Abcam ago1
Additional data
Additional Notes
In the figure, on the left is human endothelial cells lysates and on the right is bovine endothelial cells lysates.
The quality of the blot should be able to be optimized (reduced background) with some modification.
This antibody seems also recognize bovine Ago3 in Western blot.
The quality of the blot should be able to be optimized (reduced background) with some modification.
This antibody seems also recognize bovine Ago3 in Western blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 31 2010