Anti-AGO3 抗体 (ab3593)
Key features and details
- Rabbit polyclonal to AGO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-AGO3 antibody
AGO3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AGO3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human
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免疫原
Synthetic peptide corresponding to Human AGO3 aa 350-450.
(Peptide available asab24437) -
特記事項
Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).This product was previously labelled as Argonaute 3 / eIF2C 3
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3593の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
|
| ICC/IF | (1) |
1/200.
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
|
ICC/IF
1/200. |
ターゲット情報
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機能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. -
配列類似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在
Cytoplasm > P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 192669 Human
- Entrez Gene: 214150 Mouse
- Entrez Gene: 313593 Rat
- Omim: 607355 Human
- SwissProt: Q9H9G7 Human
- SwissProt: Q8CJF9 Mouse
- Unigene: 657659 Human
- Unigene: 440915 Mouse
see all -
別名
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
see all
画像
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Western blot - Anti-AGO3 antibody (ab3593)All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml
Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab3593 は 1 報の論文で使用されています。
- Hengst U et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529