製品の概要
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製品名Anti-Ago1 antibody
Ago1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Ago1
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由来種Rabbit
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アプリケーション適用あり: IP, IHC-P, WBmore details
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種交差性交差種: Mouse, Drosophila melanogaster
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) Ago1.
(Peptide available as ab23538.)
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ポジティブ･コントロール
- This antibody gave a positive signal in Drosophila embryo nuclear extract
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab5070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. PubMed: 18463631 | |
| IHC-P | Use at an assay dependent concentration. PubMed: 18528333 | |
| WB | 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa). |
ターゲット情報
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機能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
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配列類似性Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在Cytoplasm > P-body.
- Information by UniProt
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参照データベース
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 7398 Drosophila melanogaster
- Unigene: 30800 Mouse
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別名
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
画像
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Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 110 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
参考文献
This product has been referenced in:
- Mondal T et al. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS One 13:e0190548 (2018). Read more (PubMed: 29385168) »
- Zhang X et al. Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle ofAedes aegypti. Proc Natl Acad Sci U S A 114:E1895-E1903 (2017). Read more (PubMed: 28223504) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"