Anti-Ago1 抗体 (ab5070)
Key features and details
- Rabbit polyclonal to Ago1
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Ago1 antibody
Ago1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ago1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Drosophila melanogaster
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab23538) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
ターゲット情報
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機能
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
配列類似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在
Cytoplasm > P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 36544 Drosophila melanogaster
- Entrez Gene: 236511 Mouse
- SwissProt: Q8CJG1 Mouse
- Unigene: 7398 Drosophila melanogaster
- Unigene: 30800 Mouse
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別名
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
画像
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Western blot - Anti-Ago1 antibody (ab5070)Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous AbreviewAnti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDaBlot was blocked with 5% Milk for 1 hour at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (38)
ab5070 は 38 報の論文で使用されています。
- Adiliaghdam F et al. A Requirement for Argonaute 4 in Mammalian Antiviral Defense. Cell Rep 30:1690-1701.e4 (2020). PubMed: 32049003
- Goh E & Okamura K Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes. Nucleic Acids Res 47:3101-3116 (2019). PubMed: 30590701
- Wessels HH et al. Global identification of functional microRNA-mRNA interactions in Drosophila. Nat Commun 10:1626 (2019). PubMed: 30967537
- Mondal T et al. Two way controls of apoptotic regulators consign DmArgonaute-1 a better clasp on it. PLoS One 13:e0190548 (2018). PubMed: 29385168
- Zhou L et al. Importance of miRNA stability and alternative primary miRNA isoforms in gene regulation during Drosophila development. Elife 7:N/A (2018). PubMed: 30024380
