Application
ELISA
Sample
Human Recombinant protein (Part of aggrecan peptide 41AA)
Specification
Part of aggrecan peptide 41AA
Type
Indirect
Other product details
Dilution
1/250000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Pierce car#31430
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The concentration that we use is very low (1/250000), but it works well. We have make experimentations with larger quantity of BC3, but we observe signal saturation. I will add a standard curve as soon as possible.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 29 2006