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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-Aggrecan ARGxx 抗体 [BC-3] (ab3773)

  • Datasheet
Reviews (1)Q&A (20)References (53)

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [BC-3] to Aggrecan ARGxx
  • Suitable for: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WB
  • Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Isotype: IgG1

こちらの製品もご検討ください

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Anti-Collagen II antibody (ab34712)

関連製品

製品の概要

  • 製品名

    Anti-Aggrecan ARGxx antibody [BC-3]
  • 製品の詳細

    Mouse monoclonal [BC-3] to Aggrecan ARGxx
  • 由来種

    Mouse
  • 特異性

    Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes).
  • アプリケーション

    適用あり: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WBmore details
  • 種交差性

    交差種: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
    非交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Aggrecan ARGxx.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.


    Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 3.16% Tris HCl
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • 一次抗体 備考

    Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
  • ポリ/モノ

    モノクローナル
  • クローン名

    BC-3
  • ミエローマ

    x63-Ag8.653
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Aggrecan
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Recombinant Human Aggrecan protein (ab114254)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3773の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration. PubMed: 24312401
ELISA (1)
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675
IHC-Fr
Use at an assay dependent concentration.

This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections.

IHC-P
Use at an assay dependent concentration.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections.

WB
Use at an assay dependent concentration.

Detects a variety of epitopes between 50 and 250 kDa.

特記事項
ICC/IF
Use at an assay dependent concentration. PubMed: 24312401
ELISA
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675
IHC-Fr
Use at an assay dependent concentration.

This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections.

IHC-P
Use at an assay dependent concentration.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections.

WB
Use at an assay dependent concentration.

Detects a variety of epitopes between 50 and 250 kDa.

ターゲット情報

  • 関連性

    Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.
  • 細胞内局在

    Secreted
  • 参照データベース

    • Entrez Gene: 280985 Cow
    • Entrez Gene: 403828 Dog
    • Entrez Gene: 176 Human
    • Entrez Gene: 397255 Pig
    • Entrez Gene: 58968 Rat
    • Omim: 155760 Human
    • SwissProt: P13608 Cow
    • SwissProt: Q28343 Dog
    • SwissProt: P16112 Human
    • SwissProt: P07897 Rat
    see all
  • 別名

    • AGC1 antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • CSPCP antibody
    • CSPG1 antibody
    • MSK16 antibody
    see all

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (53)

ab3773 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3773 は 53 報の論文で使用されています。

  • Park SS  et al. Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex. BMC Neurosci 21:16 (2020). PubMed: 32334536
  • Novais EJ  et al. Comparison of inbred mouse strains shows diverse phenotypic outcomes of intervertebral disc aging. Aging Cell 19:e13148 (2020). PubMed: 32319726
  • Cui S & Zhang L circ_001653 Silencing Promotes the Proliferation and ECM Synthesis of NPCs in IDD by Downregulating miR-486-3p-Mediated CEMIP. Mol Ther Nucleic Acids 20:385-399 (2020). PubMed: 32203911
  • Sirong S  et al. Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis. Bone Res 8:6 (2020). PubMed: 32047705
  • Tessier S  et al. Arp2/3 inactivation causes intervertebral disc and cartilage degeneration with dysregulated TonEBP-mediated osmoadaptation. JCI Insight 5:N/A (2020). PubMed: 31961823
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 21 Abreviews or Q&A

ELISA abreview for Anti-Aggrecan ARGxx antibody [BC-3]

Excellent
Abreviews
Abreviews
Application
ELISA
Sample
Human Recombinant protein (Part of aggrecan peptide 41AA)
Specification
Part of aggrecan peptide 41AA
Type
Indirect
Read More

Abcam user community

Verified customer

投稿 Nov 29 2006

Question

Ich habe 2 Fragen zu ihren Antikörpern es geht um die Antikörper ab3777 Aggrecan xxPen antibody (BC-4) mit der lot Nummer: 204316 und um den BC-3 Antikörper ab3773 lot Nummer 194564.


Frage ich würde gerne wissen in was für einer Konzentration liegt der Antikörper vor ?

Und gibt es zufällig eine Immunglobulin Konzentration ?







Read More

Abcam community

Verified customer

Asked on Feb 27 2013

Answer



Nicht aufgereinigte Antikörper wie Zellkulturüberstand, Vollserum oder Aszites haben keine determinierte Konzentration und können stark in Ihrer Konzentration schwanken.

Wenn die spezifische Antikörperkonzentration unbekannt ist, werfen Sie einen Blick auf die „Ungefähre Konzentration“ in unserer Tabelle in den FAQs, um den Wert abschätzen zu können. Bitte beachten Sie, dass diese Konzentrationen als Richtlinien benutzt werden sollten und die verwendeten Antikörperkonzentrationen daher Ihren Resultaten angepasst werden müssen.

Laut der Tabelle ist die ungefähre Immunglobulin- Konzentration 1 - 3 mg/ml.

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Abcam Scientific Support

Answered on Feb 27 2013

Question

TSC - was cultured in serum?
is the Ab available in purified form?

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Abcam community

Verified customer

Asked on Mar 12 2012

Answer

TSC - was cultured in serum?
A: Serum was used during culture.

is the Ab available in purified form?
A: We do not routinely purify the antibody and so cannot give an answer to the second question. However, it could be purified.

I hope this is helpful. Please contact us again if you have any further questions.

Read More

Abcam Scientific Support

Answered on Mar 12 2012

Question

Dear technical team, Our customer would like to purchase 5 antibodies from abcam and wants to check control tissue. Pleaselet me knowabcam's product information. ab66025 ab102711 ab3773 ab2426 ab58632 Best regards,

Read More

Abcam community

Verified customer

Asked on Jan 06 2012

Answer

Thank you for your inquiry. Please find a list of possible positive controls below: ab2426: skin, lymph nodes Click here (or use the following: https://www.abcam.com/PCNA-antibody-ab2426.html). ab3773: cartilage samples Click here (or use the following: https://www.abcam.com/Aggrecan-ARGxx-antibody-BC-3-ab3773.html). ab58632: Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Click here (or use the following: https://www.abcam.com/Collagen-X-antibody-ab58632.html). ab66025: The recommended positive control tissues are rat head tissue, rat embryo tissue, human osteosarcoma tissue, human glioma tissue, human intestinal cancer tissue. Click here (or use the following: https://www.abcam.com/N-Cadherin-antibody-NC-17-ab66025.html). ab102711: Specifically expressed in osteoblasts Click here (or use the following: https://www.abcam.com/RUNX2-antibody-ab102711.html). I hope this information is helpful for your customer. I wish them good luck with their experiments.

Read More

Abcam Scientific Support

Answered on Jan 06 2012

Question

Which antibody is most suitable for detecting full length aggregan protein in goat tissue?

Read More

Abcam community

Verified customer

Asked on Jun 05 2007

Answer

I have been informed of the following information which I hope will help you: Ab3773: this should be reactive with goat since this antibody recognises a sequence ARG... that is conserved in all species Guinea Pig/rat to human but not mouse for some reason where the sequence is ALG.... This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Ab3778: this antibody recognises a linear sequence ..EPEEP... that is present in the IGD protein core of human and bovine aggrecan but not pig and several other animals; it seems it is not present in goat protein after BLASTing the sequence in goat. This antibody recognises the linear epitope ...EPEEP... in both intact and matrix protease degraded aggrecan from human and bovine cartilage. Please do not hesitate to contact me if I can be of further help,

Read More

Abcam Scientific Support

Answered on Jun 05 2007

Question

I want to buy a mouse monoclonal antibody to aggrecan ARGxxx Cat no. ab3773. I wang to use it for immunohistochemistry for aggrecan of rattus chondrocyte. I want to konw whether I must treat the chondrocytes with digestion mixture ( Chondroitnase ABC Sigma, Keratanase Seikagaku and keratanase II Seikagaku ) as recommended before immunohistochemistry or western-blot.

Read More

Abcam community

Verified customer

Asked on May 24 2007

Answer

Thank you for your enquiry. The following reference gives details of the protocol used with this antibody in immunohistochemistry: Roberts S et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine 25:3005-13 (2000). PubMed: 11145811 I have added the relevant protocol to our online datasheet for your convenience and include below the following publications which have all used this product. This product has been used in: Karsdal MA et al. Induction of increased cAMP levels in articular chondrocytes blocks matrix metalloproteinase-mediated cartilage degradation, but not aggrecanase-mediated cartilage degradation. Arthritis Rheum 56:1549-58 (2007). PubMed: 17469134 Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). PubMed: 12009333 Flannery CR et al. Autocatalytic cleavage of ADAMTS-4 (Aggrecanase-1) reveals multiple glycosaminoglycan-binding sites. J Biol Chem 277:42775-80 (2002). PubMed: 12202483 Malfait AM et al. Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage. J Biol Chem 277:22201-8 (2002). PubMed: 11956193 Roberts S et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine 25:3005-13 (2000). PubMed: 11145811 Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000). PubMed: 10963994 Billington CJ et al. An aggrecan-degrading activity associated with chondrocyte membranes. Biochem J 336 ( Pt 1):207-12 (1998). PubMed: 9806902 Buttle DJ et al. "Aggrecanase" activity is implicated in tumour necrosis factor alpha mediated cartilage aggrecan breakdown but is not detected by an in vitro assay. Mol Pathol 50:153-9 (1997). PubMed: 9292151 Hughes CE et al. Monoclonal antibodies that specifically recognize neoepitope sequences generated by 'aggrecanase' and matrix metalloproteinase cleavage of aggrecan: application to catabolism in situ and in vitro. Biochem J 305 ( Pt 3):799-804 (1995). PubMed: 7531436 This clone has been used in: Molecular Approaches to Osteoarthritis Research. Abstracts of the Kennedy Institute of Rheumatology Symposium, London, United Kingdom, April 18-20, 2004. Scand J Rheumatol Suppl :1-41 (2005). PubMed: 15864865 Roberts S et al. Matrix turnover in human cartilage repair tissue in autologous chondrocyte implantation. Arthritis Rheum 44:2586-98 (2001). PubMed: 11710715 Aplin JD et al. Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity. Glycobiology 8:269-76 (1998). PubMed: 9451036

Read More

Abcam Scientific Support

Answered on May 24 2007

Question

Can you tell me the molecular weigth for the full-length protein and also the protein after it has been degraded? I am using rabbit and guinea pig tissue.

Read More

Abcam community

Verified customer

Asked on Nov 03 2006

Answer

Thank you for your patience. I can see that my colleague has also written to you and referred you to the paper in which the antibody was tested, i.e. Little CB et al., Matrix Biol 21:271-88 (2002). In summary the antibody detects a variety of epitopes between 50 and 250 kDa. I also contacted the originator of the antibody. Using ab3773 in Western blots can provide a wide range (of MW) of bands that are immuno-positive for the BC-3 neoepitope (i.e. ARG... as a result of IGD aggrecanase cleavage). Thus, if you are looking at media from cartilage explant cultures exposed to cytokines such as IL-1 you will find a wide range of MW product usually from 50kD - 250kD. However, if you use cartilage from young animals this can give mainly large MW bands and if the aggrecanase activity has been low you may see only bands at 40/50 - 90kD. I hope this additional information helps you.

Read More

Abcam Scientific Support

Answered on Nov 07 2006

Question

Can you let me know the detected band size using ab3773 for synovial fluid Western blot?

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Abcam community

Verified customer

Asked on Nov 03 2006

Answer

The antibody ab3773 was tested in western blot analysis on proteoglycan metabolites released into the culture media and extracted from the cartilage matrix in cultures of bovine nasal cartilage maintained for 21 days in the paper Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). In figure 2 you will be able to see that the antibody detected bands of different sizes and thickness depending on the absence of 50 ng/ml interleukin-1 alone (IL-1), and IL-1 plus an inhibitor of MMPs at a high dose (1 mM) or a low dose (40 nM). In summary the antibody detects a variety of epitopes between 50 and 250 kDa. Because of copyright I am not able to forward the image of the figure to you or add it to the datasheet, but I hope you will be able to access the paper to see the banding pattern observed with the antibody. I hope this information will help you,

Read More

Abcam Scientific Support

Answered on Nov 06 2006

Question

With regards to the poor immunostaining of aggrecan by ab3773 product, I would like to enquire on the following. I am interested in immunostaining of pig tissues, frozen sections ( Antibodies raised in mouse or rabbit ). I am considering the 2 other abcam aggrecan antibodies ab3776 and ab3777. Hence I would like to confirm with you on the effectiveness of these antibodies for my application. Moreover I would like to have the protocols which are tested to be effective when these antibodies are used.

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Abcam community

Verified customer

Asked on May 08 2006

Answer

Thank you for getting back in touch with me. I have been in touch with the originator of this antibody. They have provided me with some further details. They mention that both ab3773 & ab3776 are not usually used for IHC studies of articular cartilage because aggrecan catabolites with these (aggrecanase ab3773 or ab3776) generated neoepitopes are not retained within the cartilage; i.e. They are usually released into the synovial fluid or media if cultures are being examined. It may be possible that some ab3773 & ab3776 positive catabolites may be retained in tendon or intervertebral disc tissue; however, we have not examined this. If this was to be undertaken on these non-cartilagenous tissues a dilution of 1:100 should suffice. Regarding using ab3777 (anti ...PEN) on cartilage; a dilution of 1:100 (same as Western blots) should suffice. If they are looking for aggrecanase-generated neoepitope then they should use ab3775 (anti- ...EGE). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on May 11 2006

Question

In the technical data sheet, it is as follows : Notes Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Furthermore, controls were done whereby no deglycosylation was carried out and the results were negative. Those with deglycosylation were faint. These were cartilage sections which were meant to be positive controls. The following dilutions were tried : 20, 50 and 100 times. Thank you.

Read More

Abcam community

Verified customer

Asked on Apr 26 2006

Answer

Thank you for getting back to me and highlighting these details on our datasheet. I can now appreciate the deglycosylation that you have performed in line with the following publication. Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). PubMed: 12009333. I am sorry to heat that you have been having difficulties with this antibody. I would like to offer you credit against your initial purchase provided it was made within the past 90 days. If this is the case please e-mail me details of the order number and date of purchase.

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Abcam Scientific Support

Answered on May 02 2006

1-10 of 21 Abreviews or Q&A

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