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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Aggrecan
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リコンビナント

Anti-Aggrecan 抗体 [6-B-4] (ab3778)

  • Datasheet
  • SDS
Reviews (5)Q&A (12)References (87)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
  • Anti-Aggrecan antibody [6-B-4] (ab3778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6-B-4] to Aggrecan
  • Suitable for: WB, IHC-P
  • Reacts with: Human

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Aggrecan protein (ab114254)
一次抗体
Product image
Anti-Collagen II antibody [2B1.5] (ab185430)
一次抗体
Product image
Anti-SOX9 antibody [EPR14335-78] (ab185966)

関連製品

製品の概要

  • 製品名

    Anti-Aggrecan antibody [6-B-4]
    Aggrecan 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [6-B-4] to Aggrecan
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • ポジティブ・コントロール

    • WB: Recombinant human Aggrecan protein. IHC-P: Human cartilage sections.
  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 08th October 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 一次抗体 備考

    This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.
  • ポリ/モノ

    モノクローナル
  • クローン名

    6-B-4
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Aggrecan
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis

関連製品

  • Alternative Versions

    • Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Recombinant Human Aggrecan protein (ab114254)
  • Recombinant Protein

    • Recombinant Human Aggrecan protein (ab114254)

アプリケーション

Our Abpromise guarantee covers the use of ab3778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 250 kDa.
IHC-P Use a concentration of 0.05 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • 細胞内局在

    Secreted; extracellular matrix.
  • 参照データベース

    • Entrez Gene: 176 Human
    • Omim: 155760 Human
    • SwissProt: P16112 Human
    • 別名

      • ACAN antibody
      • AGC 1 antibody
      • AGC1 antibody
      • AGCAN antibody
      • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
      • Aggrecan 1 antibody
      • Aggrecan core protein antibody
      • Aggrecan proteoglycan antibody
      • Aggrecan structural proteoglycan of cartilage antibody
      • Aggrecan1 antibody
      • ATEGQV antibody
      • Cartilage specific proteoglycan core protein antibody
      • Chondroitin sulfate proteoglycan 1 antibody
      • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
      • Chondroitin sulfate proteoglycan core protein 1 antibody
      • CSPG 1 antibody
      • CSPG1 antibody
      • CSPGCP antibody
      • JSCATE antibody
      • Large aggregating proteoglycan antibody
      • mcspg antibody
      • mgsk16 antibody
      • MSK 16 antibody
      • MSK16 antibody
      • SEDK antibody
      see all

    画像

    • Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)
      Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

      ab3778 was shown to react with recombinant human Aggrecan protein in western blot. 

      Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

      Secreted staining of cartilage cells.

      Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

      No staining in negative control tissue (human normal liver).

      Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    • Anti-Aggrecan antibody [6-B-4] (ab3778)
      Anti-Aggrecan antibody [6-B-4] (ab3778)

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (87)

    ab3778 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3778 は 87 報の論文で使用されています。

    • Li L  et al. XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol 235:281-293 (2020). PubMed: 31215024
    • Hernandez PA  et al. Early-Onset Osteoarthritis originates at the chondrocyte level in Hip Dysplasia. Sci Rep 10:627 (2020). PubMed: 31953438
    • Gu L  et al. Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering. Regen Biomater 7:195-202 (2020). PubMed: 32296538
    • Lei J  et al. Proteomic analysis of knee cartilage reveals potential signaling pathways in pathological mechanism of Kashin-Beck disease compared with osteoarthritis. Sci Rep 10:6824 (2020). PubMed: 32322000
    • Ledo AM  et al. Extracellular matrix mechanics regulate transfection and SOX9-directed differentiation of mesenchymal stem cells. Acta Biomater 110:153-163 (2020). PubMed: 32417266
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 17 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.15% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rhesus monkey Tissue sections (Ovarian Section)
    Specification
    Ovarian Section
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 22 2013

    Question

    send protocol for ELISA

    Read More

    Abcam community

    Verified customer

    Asked on Sep 24 2012

    Answer



    Sorry but we don't have a standard ELISA procedure for use with this antibody. However I would recommend using an indirect ELISA approach. This is outlined here https://www.abcam.com/index.html?pageconfig=resource&rid=11389

    Read More

    Abcam Scientific Support

    Answered on Sep 24 2012

    Question

    Dear Sir,
    The customer will order ab3778, ab3092, ab97022 for Flow cytometry application, sample is human PBMC. So,she would like to abcam guide for Flow cytometry method ( staining step?, wash step? )

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Thank you for contacting us.

    Unfortunately the indicated primary antibodies have not been tested with Flow Cytometry, and therefore are not covered by our Abpromise guarantee.

    I would instead recommend using a different antibody against Collagen II that has been tested in this application:

    https://www.abcam.com/Collagen-II-antibody-ab116242.html

    I am afraid none of the antibodies against Aggrecan in our catalogue have been tested in Flow Cytometry, but if you wish to test them and share your results with us (through the Abreview system) we will be happy to offer a discount code for a future purchase.

    If you would like to participate in the Abreview system and test ab3778 with Flow Cytometry, please let me know before placing the order so I can raise the discount code for you.

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews

    Ab97022 is a suitable secondary for recognising Mouse primaries with Flow Cytometry. The only recommendation for this particular antibody is using it at dilutions 1/50-1/200. For general Flow Cytometry protocols I would encourage you to have a look at our Website’s protocols which may be very helpful (open the scroll down menu under the Flow Cytometry / FACS tab to have access to all the FC protocols).

    https://www.abcam.com/index.html?pageconfig=popular_protocols

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

    Read More

    Abcam Scientific Support

    Answered on Apr 10 2012

    Question

    Welcher Antikoerper ist geignet fuer Ziege und Human, IHC-P: Versican, SOX5, Aggrecan?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Vielen Dank für Ihren Anruf.



    Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hohe Übereinstimmung mit dem Ziegen Protein hat, nur haben wir leider keinen passenden Antikörper.

    Versican und SOX5 weisen leider gar keine Übereinstimmung mit dem Ziegenprotein auf.

    Da alle unsere Aggrecan,Versican und SOX5Antikörper gegen das humane Protein entwickelt worden sind, kannich Ihnen leider keinen Vorschlag machen.

    Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Apr 10 2012

    Question

    I want to use this antibody for immunofluorescenceon my human cell samples: i) monolayer on coverslip and ii) frozen sections, both previously fixed with 2% PFA. Is there any literature that have the same application using this antibody? I would like to take reference from the protocol they used, including the details like the antibody dilution ratio and the antigen retrieval steps). I would be grateful if you can refer to me the NCBI code of these papers and suggest a suitable protocol. Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    Thank you for your inquiry.


    We have quite a few references for this antibody to be used in ICC/IF and IHC-Fr/IHC-P. You can find them listed here and they are linked to the PubMed IDs.


    https://www.abcam.com/Aggrecan-antibody-6-B-4-ab3778-references.html


    You may also be interested in reading our customer reviews, called Abreviews, which include their protocol and images.


    https://www.abcam.com/Aggrecan-antibody-6-B-4-ab3778-reviews.html


    I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Question

    Por favor, indiquenos unos anticuerpos validos para la siguiente situacion:
    Applications: Immunochemestry
    Reacts with: Chondrocytes or precursors of chondrocytes
    Sample: canine (dog) trachea
    Muchas gracias

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2012

    Answer

    Gracias por contactarnos.

    Varios marcadores pueden usarse para la detección de condrocitos mediante immunohistoquímica. Solo algunos de ellos se han testado en especie canina, el resto me temo que no se han testado, por lo que se desconoce su funcionamiento en ellas.

    Le indico algunas posibilidades con los números de catálogo de Abcam:

    Collagen II: podría usarse ab300 o ab34712. Ninguno de ellos se ha testado en perro. Si está interesado en usarlo en esta especie, quizás le interese participar en el sistema Abreviews de evaluación de anticuerpos, por el cual les ofrecemos un anticuerpo completamente gratuito al mandarnos los resultados de testar el anticuerpo en la nueva especie. Si le interesa, por favor, comuníquemelo antes de realizar el pedido.

    Para saber más sobre el sistema Abreviews: https://www.abcam.com/abreviews

    Aggrecan: ab3778. Tampoco testado en perro, pero susceptible de evaluar su aplicación.

    CD29: ab30388. En este caso tampoco hay datos de su uso en especie canina, tendría que evaluarlo usted misma.

    CD44: ab19622 y ab119863. Ninguno de ellos se ha testado en perro, sin embargo, debido al elevado porcentaje de homología con dicha especie, es probable que reaccione. Por ello, si le interesa comprobarlo, puede igualmente usarlo y enviarnos los resultados, beneficiándose de una futura compra totalmente gratuita.

    SOX9: ab26414 y ab71762. En este caso, ambos anticuerpos se han usado y caracterizado en especie canina, por lo que están cubiertos por la garantía Abpromise.

    Espero que esta información le sea de utilidad. Si necesita que le enviemos una proforma de alguno o varios de los anticuerpos, si necesita más información de alguno de ellos, o si quiere saber más acerca del sistema Abreview, por favor, no dude en volvernos a contactar.

    Read More

    Abcam Scientific Support

    Answered on Jan 31 2012

    Question

    Please send the antigen retrieval protocol for the aggecan antibody ab3778 and the collagen II antibody ab34712.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2012

    Answer

    Thank you for contacting us.

    For the aggrecan antibody ab3778, I recommend the following two papers, if they are avalable to you, the first of which describes production of this clone and its use for IHC. We do not have access to the journal.

    Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000). PubMed: 10963994

    Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). PubMed: 12009333

    Please also note the review of ab3778 submitted by a customer on March 21, 2007, who included this note:

    "We tried multiple retreival methods and found that pronase and hyaluronidase will work. The best results were with hyaluronidase".

    (I suggest trying the hyaluronidase solution that is mentioned below for use with the collagen II antibody: 2mg/ml hyaluronidase in TBS for 2 hours at 37C).

    For the collagen II antibody, an Abcam customer submitted an Abreview with the following protocol for IHC of formalin-fixed paraffin-embedded human cartilage tissue:

    Antigen retrieval step: Enzymatic - Buffer/Enzyme Used: 2mg/ml hyaluronidase with 0.125U/ml chondroitinase in TBS for 2hrs 37C.

    Blocking step: Normal goat serum in 1% BSA as blocking agent for 30 mins · Concentration: 20 % Temperature: 21°C.

    Dilution of antibody: 1/50

    Incubation time: 18 hours

    Temperature: 4°C

    Diluent: Blocking solution.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2012

    Question

    LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse cartilage PRIMARY ANTIBODY Dilution 1/50 in PBS/BSA 1% for 1 hour at RT Wash with PBS DETECTION METHOD DAB ANTIBODY STORAGE CONDITIONS Short term at 4°C Long term at -20°C or -80°C FIXATION OF SAMPLE Paraformaldehyde 24hours ANTIGEN RETRIEVAL Enzymatic method: Hyaluronidase PERMEABILIZATION STEP no BLOCKING CONDITIONS BSA 1% 30min at 37°C SECONDARY ANTIBODY Vectastain ABC kit HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES In the scientific support, in your answer at the question 4 you say that these Ab is only react with human and cow! But in the data sheet you say that it react with mouse???

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2011

    Answer

    Thank you very much for your email. Indeed, I have to agree with you that there is a problem. I would like to apologize for this and would like to reassure you that we will investigate this further and take the appropriate actions. The mouse reactivity has been added due to a customer feedback. Doing however the alignment of the immunogen with the mouse sequence, there is little overlap and it looks therefore rather tricky to get a specific staining on mouse cells. ** *.:*:***: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111207-093227-0400-33224393-pg I will remove the mouse reactivity and replace this antibody for you with another antibody of your choice. Please do provide me therefore with the order details, such as the order number or the order date and name of the person placing the order. Unfortunately, we do have currently however no anti aggrecan antibody tested in your application and species. If you would like nevertheless to use one of our other anti aggrecan antibodies, I would be pleased to make predictions for the suitability. I apologize again for this error and am looking forward to hear back from you.

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aggrecan antibody [6-B-4]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEF, R1 ES cells, E14 ES cells)
    Specification
    MEF, R1 ES cells, E14 ES cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 20 2011

    Question

    Do you have the certificate of analysis for Lot#:196340、Lot#:346985、Lot#:393795?

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2008

    Answer

    Thank you for your enquiry. Unfortunately, we do not have a Certificate of Analysis for this product. If there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on May 07 2008

    1-10 of 17 Abreviews or Q&A

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