Application
Immunoprecipitation
Immuno-precipitation step
Other - ab1229
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Treatment
transfected with plasmid DNA
Total protein in input
5e+006 cells
Other product details
Concentration
2 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Name
Non-Abcam antibody was used: Anti-V5 pAb
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Additional data
Additional Notes
PVDF membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Christina Engel
Verified customer
投稿 Oct 10 2013