Agarose Anti-6X His tag® 抗体 (ab1231)

Submit a review Q&A (2)References (1)

Key features and details

  • Agarose Goat polyclonal to 6X His tag®
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

製品の概要

  • 製品名

    Agarose Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧

  • 製品の詳細

    Agarose Goat polyclonal to 6X His tag®

  • 由来種

    Goat

  • 標識

    Agarose

  • アプリケーション

    適用あり: IPmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Full length native

    HHHHHH

    (6-His)(purified): conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.

ターゲット情報

  • 関連性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • 細胞内局在

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • 別名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献 (1)

ab1231 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1231 は 1 報の論文で使用されています。

  • Ma X  et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Ich habe bei Ihnen das Produkt ab 1231 erworben und hätte zu diesem
Produkt einige Fragen (Anti-6XHistag antibody, ..). Ich
möchte, wenn möglich, ein Proteinpartikel pro Bead anbringen. Hierfür
bräuchte ich die Konzentration der Beadpartikel/mL Lösung. Daher wäre
ich Ihnen sehr verbunden, wenn Sie mir diese mitteilen könnten.
Darüber hinaus würde ich es sehr zu schätzen wissen, wenn Sie mir
ungefähr die Größe eines Beadpartikels mitteilen könnten.

Read More
Answer

Vielen Dank fürIhre Anfrage.

Das Labor hat bestätig,dass in 400ul Puffer (50% Slurry)es 200ul Beads hat(und 100ugAntikörper).

Hier ist ein Link zu den Beads, welchebenutzt wurden. Ich bin mir nicht sicher, wie Sie genau die Anzahl Partikel berechnen möchten/können. Bitte lassen Sie mich wissen, falls Sie mehr Informationen vom Labor bräuchten:...

Ich hoffe, dies hilft Ihnen trotzdemweiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Question

What type of agarose beads were used?

Read More
Answer

The type of agarose beads that were used were - Uniflow 4 from Sterogene.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com