Anti-Aflatoxin B1 抗体 [AFA-1] (ab1017)

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Key features and details

  • Mouse monoclonal [AFA-1] to Aflatoxin B1
  • Suitable for: ELISA
  • Reacts with: Species independent
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Aflatoxin B1 antibody [AFA-1]

  • 製品の詳細

    Mouse monoclonal [AFA-1] to Aflatoxin B1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Purified Aflatoxin B1

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 5th December 2019. Lot numbers higher than GR285242 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.20
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    AFA-1

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals. Aflatoxins are well recognized as a cause of liver cancer, but they have additional important toxic effects. Aflatoxin B1 is a potent hepatocarcinogenic and mutagenic mycotoxin of Aspergillus flavus.

  • 別名

    • AFB1 antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献

ab1017 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1017 は論文での使用が確認できていません。

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1-4 of 4 Abreviews or Q&A

Question

Thanks for reply, pardon my further question, what about 250 µg? what's it represent?

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Answer

This item is sold in a 250ug size. The stock concentration of the antibody is 1mg/ml. Therefore each vial purchased will contain 250ul of antibody.

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Question

Article No: AB1017

Description : Aflatoxin B1, Mouse monoclonal [AFA-1] t

Could you kindly clarify the product above is hazardous or not.

Thank you,

Regards,

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Answer

Thank you for contacting us.

The product ab1017 does not contain any hazardous chemical e.g. Sodium Azide or Thimersol so this product should not be included in hazardous chemicals. However as this an antibody so general laboratory precautionary measures should be checked while working with this product.

We also are not aware of any health hazards with this product. However please note, this product is intended to be used for research purpose only - it should not be used for diagnostic purpose.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Please send detection sensitivity of Aflatoxin B1, B2, G1 and G2 and method(s) used (e.g. 50% inhibition in competitive ELISA and incubation duration, etc.) for your Aflatoxin monoclonal antibodies or any others that are not listed on your website.
In addition to the initial inquiry, I’d be interested in knowing the carrier protein used as immunogen and in detection sensitivity evaluation.

Thanks.

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Answer

Thank you very much for contacting us with your enquiry, and for your patience while I have been in touch with the production labs for more information about our aflatoxin antibodies.

We have three monoclonal antibodies against aflatoxin: ab685, ab1017, and ab1985. Here is the information about each of these antibodies:

Ab685: reacts with AFM1 and AFB1. It was raised against AFM1-BSA conjugate, but unfortunately the lab does not have further data regarding the detection sensitivity of the antibody.

Ab1017: reacts with AFB1, and cross-reactivity with other aflatoxins is not known at this time. It was raised against purified AFB1. It has been tested in ELISA at 5 ug/mL against AFB1 coated on wells at 1 ug/mL but the detection sensitivity not been determined.

Ab1985: reacts with AFB1 and AFB2, and it does not cross-react with G1, G2, and M1. It was raised against aflatoxin from Aspergillus flavus conjugated to BSA. The lab has been able to reach the detection limit of 0.2 ng/mL againt AFB1-BSA in competitive ELISA. In this assay, the antibody incubation was performed for 1 hour at 37C and different concentrations of antibody were used.

I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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Question

I'm afraid that I don't understand so good the information that you send me. I all ready check the website that you kindly recommended me, but I still have a doubt; is this aflatoxin B1 antibody labeling with fluorescein?, I'm not sure if I didn't see this information in the datasheet. My goal is to see the Aflatoxin B1 antibody labeling under fluorescence microscopy. Thank you again ... we are in touch.

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Answer

Thank you for your email. At this time, we currently have one Aflatoxin B1 antibody, it is catalog # ab1017. The antibody in unconjugated; it is not labeled. However, we do carry a wide range of secondary antibodies which can be used with this primary, such as ab6785 - Goat polyclonal to Mouse IgG H&L (FITC). For more information regarding ab1017, please refer to the online datasheet. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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