Anti-AFF4 抗体 (ab103586)
Key features and details
- Rabbit polyclonal to AFF4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AFF4 antibody
AFF4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AFF4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein within Human AFF4 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Human and Mouse liver tissue lysates; AFF4 transfected 293T cell line lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 127 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 127 kDa. |
ターゲット情報
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関連性
May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 27125 Human
- Entrez Gene: 93736 Mouse
- Omim: 604417 Human
- SwissProt: Q9UHB7 Human
- SwissProt: Q9ESC8 Mouse
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別名
- AF4/FMR2 family member 4 antibody
- AF5Q31 antibody
- Alf4 antibody
see all
画像
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Western blot - Anti-AFF4 antibody (ab103586)Anti-AFF4 antibody (ab103586) at 1/500 dilution + Human liver tissue lysate at 50 µg
Predicted band size: 127 kDa -
Western blot - Anti-AFF4 antibody (ab103586)Anti-AFF4 antibody (ab103586) at 1/500 dilution + Mouse liver tissue lysate at 50 µg
Predicted band size: 127 kDa -
Western blot - Anti-AFF4 antibody (ab103586)All lanes : Anti-AFF4 antibody (ab103586) at 1/500 dilution
Lane 1 : AFF4 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 127 kDa
データシートおよび資料
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Datasheet download
参考文献 (4)
ab103586 は 4 報の論文で使用されています。
- Li J et al. Long noncoding RNA ZFPM2-AS1 is involved in lung adenocarcinoma via miR-511-3p/AFF4 pathway. J Cell Biochem 121:2534-2542 (2020). PubMed: 31692047
- Zheng L et al. Triptonide Modulates MAPK Signaling Pathways and Exerts Anticancer Effects via ER Stress-Mediated Apoptosis Induction in Human Osteosarcoma Cells. Cancer Manag Res 12:5919-5929 (2020). PubMed: 32765093
- Olson CM et al. Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation. Nat Chem Biol 14:163-170 (2018). PubMed: 29251720
- Zhou CC et al. AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs. Bone Res 5:17044 (2017). WB ; Human . PubMed: 28955517
