Anti-ADRA1B 抗体 [EPR10336] (ab169523)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10336] to ADRA1B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ADRA1B antibody [EPR10336]
ADRA1B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10336] to ADRA1B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human ADRA1B aa 150-250. The exact sequence is proprietary.
Database link: P35368 -
ポジティブ・コントロール
- WB: HeLa, PC-3 and NIH/3T3 cell lysate, human fetal brain lysates, mouse and rat brain lysates, mouse and rat stomach lysates; IHC-P: Rat and mouse stomach tissue. Human ovarian carcinoma, breast carcinoma, and kidney tissues.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10336 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
|
特記事項 |
---|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
ターゲット情報
-
機能
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily. -
細胞内局在
Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. - Information by UniProt
-
参照データベース
- Entrez Gene: 147 Human
- Entrez Gene: 11548 Mouse
- Entrez Gene: 24173 Rat
- Omim: 104220 Human
- SwissProt: P35368 Human
- SwissProt: P97717 Mouse
- SwissProt: P15823 Rat
- Unigene: 368632 Human
see all -
別名
- ADA1B_HUMAN antibody
- ADRA 1 antibody
- Adra 1b antibody
see all
画像
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining predominantly on collecting duct of human kidney. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/2000 dilution (Purified)
Lane 1 : Human fetal brain lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 5 : Rat brain lysates
Lane 6 : Mouse stomach lysates
Lane 7 : Rat stomach lysates
Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse stomach. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 µg/mL) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma. The section was incubated with ab169523 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 minutes.
-
All lanes : Anti-ADRA1B antibody [EPR10336] (ab169523) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : PC-3 lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (19)
ab169523 は 19 報の論文で使用されています。
- Gao X et al. Heteromerization between α1B -adrenoceptor and chemokine (C-C motif) receptor 2 biases α1B -adrenoceptor signaling: Implications for vascular function. FEBS Lett 596:2706-2716 (2022). PubMed: 35920096
- Wang J et al. Norepinephrine exposure restrains endometrial decidualization during early pregnancy. J Endocrinol 248:277-288 (2021). PubMed: 33434145
- Albee LJ et al. Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. PLoS One 16:e0253821 (2021). PubMed: 34166476
- Gao R et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
- He W et al. Alterations in the phosphodiesterase type 5 pathway and oxidative stress correlate with erectile function in spontaneously hypertensive rats. J Cell Mol Med 24:14280-14292 (2020). PubMed: 33118708