Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
製品の概要
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製品名
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
Adipogenesis キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
> 0.2 nM -
検出範囲
0.2 nM - 10 nM -
全工程の試験時間
0h 40m -
製品の概要
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.
Visit our FAQs page for tips and troubleshooting. -
特記事項
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests Adipogenesis Assay Buffer WM 1 x 25ml Adipogenesis Enzyme Mix Green 1 vial Adipogenesis Probe (in DMSO solution) Red 1 x 200µl Lipase Orange 1 vial Lipid Extraction Solution NM 1 x 10ml Triglyceride Standard Yellow 1 x 0.3ml -
研究分野
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関連性
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
関連製品
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Related Products
画像
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Example of a standard curve obtained with ab102513.
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Example of a standard curve obtained with ab102513.
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Glycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Glycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Glycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells
プロトコール
参考文献
This product has been referenced in:
- Li Y et al. Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells. Lipids Health Dis 16:181 (2017). Functional Studies ; Mouse . Read more (PubMed: 28946872) »
- Leow SC et al. The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. Development 143:950-61 (2016). Read more (PubMed: 26893351) »