Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 nM
製品の概要
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製品名
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
Adipogenesis キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
> 0.2 nM -
検出範囲
0.2 nM - 10 nM -
全工程の試験時間
0h 40m -
製品の概要
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K610 Adipogenesis Colorimetric/Fluorometric Assay Kit. K610-100 is the same size as the 100 test size of ab102513.
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests 2000 tests Adipogenesis Assay Buffer WM 1 x 25ml 20 x 25ml Adipogenesis Enzyme Mix Green 1 vial 20 vials Adipogenesis Probe (in DMSO solution) Red 1 x 200µl 20 x 200µl Lipase Orange 1 vial 20 vials Lipid Extraction Solution NM 1 x 10ml 20 x 10ml Triglyceride Standard Yellow 1 x 0.3ml 20 x 0.3ml -
研究分野
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関連性
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
関連製品
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Related Products
画像
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Functional Studies - Adipogenesis Detection Kit (ab102513)
Example of a standard curve obtained with ab102513.
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Functional Studies - Adipogenesis Detection Kit (ab102513)Example of a standard curve obtained with ab102513.
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Functional Studies - Adipogenesis Detection Kit (ab102513) - FluorometricGlycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Functional Studies - Adipogenesis Detection Kit (ab102513) - ColourimetricGlycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample.
Ms brain value retrieved from 1:9 dilution of original 12.4mg/mL sample.
Ms muscle value retrieved from 1:9 dilution of original 7.5mg/mL sample.
Ms heart value retrieved from 1:27 dilution of original 16.4mg/mL sample.
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Functional Studies - Adipogenesis Detection Kit (ab102513) - ColourimetricGlycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab102513 は 9 報の論文で使用されています。
- Jung YJ et al. Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes. Sci Adv 6:eaay6721 (2020). PubMed: 32232152
- Zhou Y et al. Maternal diet intervention before pregnancy primes offspring lipid metabolism in liver. Lab Invest 100:553-569 (2020). PubMed: 31748681
- Stelling E et al. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation. PLoS Biol 18:e3000739 (2020). PubMed: 33370269
- Xu H et al. A long-term maternal diet intervention is necessary to avoid the obesogenic effect of maternal high-fat diet in the offspring. J Nutr Biochem 62:210-220 (2018). PubMed: 30316166
- Li Y et al. Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells. Lipids Health Dis 16:181 (2017). Functional Studies ; Mouse . PubMed: 28946872
- Leow SC et al. The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. Development 143:950-61 (2016). PubMed: 26893351
- Llobet L et al. OXPHOS xenobiotics alter adipogenic differentiation at concentrations found in human blood. Dis Model Mech N/A:N/A (2015). Functional Studies ; Human . PubMed: 26398948
- Osiecki MJ et al. Packed Bed Bioreactor for the Isolation and Expansion of Placental-Derived Mesenchymal Stromal Cells. PLoS One 10:e0144941 (2015). PubMed: 26660475
- Joseph R et al. ACSL1 Is Associated With Fetal Programming of Insulin Sensitivity and Cellular Lipid Content. Mol Endocrinol 29:909-20 (2015). PubMed: 25915184