Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513)

I am happy to confirm that tissue culture plates can sustain 90°C and can therefore be used without problem in this experiment.

In our hands, ˜1,000-10,000 differentiated 3T3 cells using 100ul Lipid Extraction Solution are sufficient for the colorimetric assay. Then we use 5-50ul of the extract for the assay. I would start with 5ul of the lipid extract for the assay. If this is too much, then I would do a pilot experiment using several dilutions to see which one gives me the best results within the linear range of the std. curve.


The protocol you mention is correct:

After culturing and treating your cells in a 6 or 12 well plate, you should remove the medium, wash with PBS, add 100ul lipid extraction buffer, heat for 30 mins, cool to RT and then use the liquid for assay. You can spin down to remove any debris.

The lipid extraction buffer can be thawed at RT.

Ibelieve the standard can be made available separately fromthe kit. If it is these things usually take 1-2 weeks to become available for purchase. And since itis a new product it would take 1-2 weeks after that for us to acquire stock and ship to you. Please let me know if you are interested.
To normalize with total protein, please use buffers like RIPA for lysis.
Hope this information has been helpful for you. Please let me know if you have any other questions.

I'm happy to help! Please let me know if you need anything further.

Thank you for your enquiry.
At this time custom conjugation services are not available.
Sorry I could not be more helpful at this time. Please contact me again if you have any further questions.

ab102513
Sample types: Cell and Tissue culture supernatants
Lipid extraction performed: The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
ab65336:
Samples: Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
No specific lipid extraction.
I hope this is helpful. Please contact me again if you have any further questions.

Thank you for calling Abcam.

The sample volume number that you use is the volume that you originally added to the reaction at stage 1 (add 5-50ul of sample). To normalize the data, you can either divide the value you get by the number of cells you started out with or the amount of tissue.

If there is anything else I can help you with, please let me know.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry about the problems caused by this product. It is a good rule of thumb to always thoroughly read the protocols and call us if you have any questions at all. I would also remind you that this product only has a 6 month guarantee and shelf life. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.