Anti-ADFP 抗体 [ADFP/1366] - BSA and Azide free (ab269759)
Key features and details
- Mouse monoclonal [ADFP/1366] to ADFP - BSA and Azide free
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-ADFP antibody [ADFP/1366] - BSA and Azide free
ADFP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ADFP/1366] to ADFP - BSA and Azide free -
由来種
Mouse -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Recombinant fragment within Human ADFP aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q99541 -
ポジティブ・コントロール
- Human adrenal tissue
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特記事項
ab269759 is a carrier free version of ab219299. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
ADFP/1366 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
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IHC-P |
Human
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アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
Protein Array |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
ターゲット情報
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機能
May be involved in development and maintenance of adipose tissue. -
組織特異性
Milk lipid globules. -
配列類似性
Belongs to the perilipin family. -
翻訳後修飾
Acylated; primarily with C14, C16 and C18 fatty acids. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q99541 Human
- Unigene: 3416 Human
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別名
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
画像
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ab219299 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219299).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269759 は論文での使用が確認できていません。