製品の概要

  • 製品名
    Anti-Adenovirus Type 5 antibody
  • 製品の詳細
    Rabbit polyclonal to Adenovirus Type 5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Neutralising, IHC-P, IHC-Fr, ELISA, WB, ICC/IFmore details
  • 免疫原

    Purified Adenovirus type 5

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml).
IHC-P Use at an assay dependent concentration. PubMed: 20442708
IHC-Fr Use at an assay dependent concentration. PubMed: 20442708
ELISA Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
  • 別名
    • Adenovirus 5 antibody
    • AdV 5 antibody

画像

  • ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

    See Abreview

  • Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

    Bottom blot (B): As above, except ab6982 omitted.

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
  • Additional information regarding Adenovirus type 5 capsid proteins. 
  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    All lanes : human hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 130 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 seconds


    Blocked with 5% milk

    See Abreview

  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : Whole cell lysate prepared from U2OS cells.
    Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit conjugated to IRDye 700DX


    Blocking step was performed using 5% milk for 1 hour at 23°C.

    See Abreview

参考文献

This product has been referenced in:
  • Sims JD  et al. Resistance to receptor-blocking therapies primes tumors as targets for HER3-homing nanobiologics. J Control Release 271:127-138 (2018). Read more (PubMed: 29288681) »
  • Martin NT  et al. Targeting polysialic acid-abundant cancers using oncolytic adenoviruses with fibers fused to active bacteriophage borne endosialidase. Biomaterials 158:86-94 (2018). Read more (PubMed: 29304405) »

See all 40 Publications for this product

レビューと Q&A

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Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
infected with Adv-iNOS
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Mar 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (AdV-infected submandibular salivary gland)
Specification
AdV-infected submandibular salivary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX 0.2%
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C
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Abcam user community

Verified customer

投稿 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
25 µg
Specification
Hek293
Treatment
AdV-infected
Gel Running Conditions
Non-reduced Denaturing (4-12% Tris-Bis)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

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投稿 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 15°C
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Abcam user community

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投稿 Aug 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (293e4)
Specification
293e4
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type
Indirect
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Abcam user community

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投稿 Jan 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
35 µg
Specification
U2OS
Treatment
infected with Adenovirus 5
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Jan 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A293 Cells)
Loading amount
300 µg
Specification
A293 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Oct 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Username

Mr. Oliver Scheibe

Verified customer

投稿 Feb 24 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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