製品の概要

  • 製品名
    Anti-Adenovirus Type 5 antibody
  • 製品の詳細
    Rabbit polyclonal to Adenovirus Type 5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Neutralising, IHC-P, IHC-Fr, ELISA, WB, ICC/IFmore details
  • 免疫原

    Purified Adenovirus type 5

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml).
IHC-P Use at an assay dependent concentration. PubMed: 20442708
IHC-Fr Use at an assay dependent concentration. PubMed: 20442708
ELISA Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
  • 別名
    • Adenovirus 5 antibody
    • AdV 5 antibody

画像

  • ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

    See Abreview

  • Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

    Bottom blot (B): As above, except ab6982 omitted.

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
  • Additional information regarding Adenovirus type 5 capsid proteins. 
  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    All lanes : human hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocked with 5% milk

    See Abreview

  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : Whole cell lysate prepared from U2OS cells.
    Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit conjugated to IRDye 700DX


    Blocking step was performed using 5% milk for 1 hour at 23°C.

    See Abreview

参考文献

This product has been referenced in:
  • Sims JD  et al. Resistance to receptor-blocking therapies primes tumors as targets for HER3-homing nanobiologics. J Control Release 271:127-138 (2018). Read more (PubMed: 29288681) »
  • Martin NT  et al. Targeting polysialic acid-abundant cancers using oncolytic adenoviruses with fibers fused to active bacteriophage borne endosialidase. Biomaterials 158:86-94 (2018). Read more (PubMed: 29304405) »
See all 46 Publications for this product

レビューと Q&A

1-10 of 31 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
infected with Adv-iNOS
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Mar 31 2017

Answer


ab6982 is a plyclonal antibody and as such shows recognition to every protein in the Ad5 capsid, including the knob.

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Answer

Thank you very much for your reply.

If the specific signal is fairly strong, you can further dilute the antibody to 1:200-1:1000 which should help reduce non-specific binding. You could also try blocking the tissue overnight at 4C, and continue to use the 1:5 serum for blocking.

We do fully guarantee this antibody to work in IHC-P, so if these suggestions aren't helpful then I'd be happy to send a replacement with our other adenovirus type 5 antibody, ab3165, which is a monoclonal and may be more specific-

https://www.abcam.com/Adenovirus-Type-5-antibody-M58-ab3165.html

We can alternatively issue a credit or refund for your original purchase. Please keep me updated about any new results with ab6982, and let me know if you'd like to receive a replacement, credit, or refund. I look forward to hearing from you. If there is anything else that we can do for you please don't hesitate to ask.

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Answer

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab to request more information about this antibody.

The antibody was validated in IHC according to the following publication, and unfortunately we haven't tested this antibody in IHC in our own labs so we haven't seen this kind of cross-reactivity-
Guedan S et al. Hyaluronidase expression by an oncolytic adenovirus enhances its intratumoral spread and suppresses tumor growth. Mol Ther 18:1275-83 (2010).
The immunogen was a pharmaceutical grade purified virus so we expected minimal cross-species recognition. The virus was grown in mammalian cells (HEK 293) so it's possible that there may be some cross recognition to other mammalian targets, but unfortunately we do not know what specific proteins the antibody would cross-react with.
It may be possible to reduce the cross-reactive signal by alterring some steps of the protocol. At what dilution are you using the antibody? What kind of blocking solution do you use? Could you describe the antigen retrieval protocol that you use?
I look forward to hearing from you. We do fully guarantee this antibody to work in IHC on paraffin-embedded tissue for up to 6 months after purchase, so I can issue a replacement, credit, or refund if you are not satisfied with the results. If you would like to accept one of these options, please let me know your original order or PO number and I'll set this up promptly.
Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (AdV-infected submandibular salivary gland)
Specification
AdV-infected submandibular salivary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX 0.2%
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C

Abcam user community

Verified customer

投稿 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
25 µg
Specification
Hek293
Treatment
AdV-infected
Gel Running Conditions
Non-reduced Denaturing (4-12% Tris-Bis)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 15°C

Abcam user community

Verified customer

投稿 Aug 24 2012

Question
Answer

Thank you for calling Abcam earlier today.

I am sorry that you are not getting any signal using the current lot of ab6982. As we discussed, I am sending you a new vial of ab6982 to use. Your new order number is */.

If you have any difficulties with the new vial of the antibody, or if there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting us.

Yes, I can confirm that "Type 5" is a shortened version of the more scientifically precise term serotype 5. They both mean exactly the same.All Adenovirus classification is based upon serotyping. This antibody ab6982,was raised against serotype 5 Adenovirus.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

We do not have this antibody inbiotinylated form, but we do have conjugation kits for biotinylation:

https://www.abcam.com/easylink

Biotin type A (e.g. ab102865 (3x10 ug) and other sizes)
https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10µg-Biotin-ab102865.html

Biotin type B (e.g. ab102868 (3x10 ug) and other sizes)
https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-3-x-10µg-Biotin-ab102868.html

Since the antibody comes as whole antiserum, it would need to be purified before the conjugation reaction, using an Antibody serum purification kit (1 (ab109208) or 3 purifications(ab109209)).

I will also check with the lab if a custome conjugation might be possible, but if it would be possible, it would require an orderof at least 10 vials. I will let you know what I find out.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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