Anti-Adenovirus Type 5 抗体 (ab6982)

ab6982 is a plyclonal antibody and as such shows recognition to every protein in the Ad5 capsid, including the knob.

Thank you very much for your reply.

If the specific signal is fairly strong, you can further dilute the antibody to 1:200-1:1000 which should help reduce non-specific binding. You could also try blocking the tissue overnight at 4C, and continue to use the 1:5 serum for blocking.

We do fully guarantee this antibody to work in IHC-P, so if these suggestions aren't helpful then I'd be happy to send a replacement with our other adenovirus type 5 antibody, ab3165, which is a monoclonal and may be more specific-

https://www.abcam.com/Adenovirus-Type-5-antibody-M58-ab3165.html

We can alternatively issue a credit or refund for your original purchase. Please keep me updated about any new results with ab6982, and let me know if you'd like to receive a replacement, credit, or refund. I look forward to hearing from you. If there is anything else that we can do for you please don't hesitate to ask.

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab to request more information about this antibody.

The antibody was validated in IHC according to the following publication, and unfortunately we haven't tested this antibody in IHC in our own labs so we haven't seen this kind of cross-reactivity-
Guedan S et al. Hyaluronidase expression by an oncolytic adenovirus enhances its intratumoral spread and suppresses tumor growth. Mol Ther 18:1275-83 (2010).
The immunogen was a pharmaceutical grade purified virus so we expected minimal cross-species recognition. The virus was grown in mammalian cells (HEK 293) so it's possible that there may be some cross recognition to other mammalian targets, but unfortunately we do not know what specific proteins the antibody would cross-react with.
It may be possible to reduce the cross-reactive signal by alterring some steps of the protocol. At what dilution are you using the antibody? What kind of blocking solution do you use? Could you describe the antigen retrieval protocol that you use?
I look forward to hearing from you. We do fully guarantee this antibody to work in IHC on paraffin-embedded tissue for up to 6 months after purchase, so I can issue a replacement, credit, or refund if you are not satisfied with the results. If you would like to accept one of these options, please let me know your original order or PO number and I'll set this up promptly.
Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

Thank you for contacting us.

Yes, I can confirm that "Type 5" is a shortened version of the more scientifically precise term serotype 5. They both mean exactly the same.All Adenovirus classification is based upon serotyping. This antibody ab6982,was raised against serotype 5 Adenovirus.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

We do not have this antibody inbiotinylated form, but we do have conjugation kits for biotinylation:

https://www.abcam.com/easylink

Biotin type A (e.g. ab102865 (3x10 ug) and other sizes)
https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10µg-Biotin-ab102865.html

Biotin type B (e.g. ab102868 (3x10 ug) and other sizes)
https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-3-x-10µg-Biotin-ab102868.html

Since the antibody comes as whole antiserum, it would need to be purified before the conjugation reaction, using an Antibody serum purification kit (1 (ab109208) or 3 purifications(ab109209)).

I will also check with the lab if a custome conjugation might be possible, but if it would be possible, it would require an orderof at least 10 vials. I will let you know what I find out.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank very much for your patience. This antibody has been developed as a serum; it is not purified further. We are unable to provide the purified version of this product. We also can’t purify it as a custom request. I am sorry we are unable to fulfil your request this time. We are however ready to provide any additional information you may need which can help you to develop your experiment. I hope this information will be helpful. Should you any further questions please do not hesitate to ask?