Anti-Adenovirus Type 5 抗体 (ab6982)
Key features and details
- Rabbit polyclonal to Adenovirus Type 5
- Suitable for: Neutralising, ELISA, WB, ICC/IF
- Isotype: IgG
製品の概要
-
製品名
Anti-Adenovirus Type 5 antibody -
製品の詳細
Rabbit polyclonal to Adenovirus Type 5 -
由来種
Rabbit -
アプリケーション
適用あり: Neutralising, ELISA, WB, ICC/IFmore details -
種交差性
交差種: Other species -
免疫原
Purified Adenovirus type 5
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Neutralising | Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
|
| ELISA | Use at an assay dependent concentration. Recognizes Ad5 immobilized on microtiter plates. |
|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
-
関連性
Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults. -
別名
- Adenovirus 5 antibody
- AdV 5 antibody
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
-
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted. -
Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)Additional information regarding Adenovirus type 5 capsid proteins. -
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous AbreviewAll lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
All lanes : human hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocked with 5% milk
-
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
プロトコール
参考文献 (59)
ab6982 は 59 報の論文で使用されています。
- Wang Z et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638
- Brugada-Vilà P et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics 10:2744-2758 (2020). PubMed: 32194832
- Saari H et al. Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. J Extracell Vesicles 9:1747206 (2020). PubMed: 32363012
- Costa R et al. Characterization of Adenovirus 5 E1A Exon 1 Deletion Mutants in the Viral Replicative Cycle. Viruses 12:N/A (2020). PubMed: 32075072
- Crisostomo L et al. Temporal dynamics of adenovirus 5 gene expression in normal human cells. PLoS One 14:e0211192 (2019). PubMed: 30677073
