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Microbiology Organism Virus DNA Virus double stranded DNA Virus Adenovirus
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Anti-Adenovirus Type 5 抗体 (ab6982)

  • Datasheet
  • SDS
Reviews (9)Q&A (20)References (59)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
  • Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)

Key features and details

  • Rabbit polyclonal to Adenovirus Type 5
  • Suitable for: Neutralising, ELISA, WB, ICC/IF
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-Adenovirus Type 5 antibody
  • 製品の詳細

    Rabbit polyclonal to Adenovirus Type 5
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Neutralising, ELISA, WB, ICC/IFmore details
  • 種交差性

    交差種: Other species
  • 免疫原

    Purified Adenovirus type 5

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Adenovirus

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab6982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising Use at an assay dependent concentration.

This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137

ELISA Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
  • 別名

    • Adenovirus 5 antibody
    • AdV 5 antibody

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
    ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

    See Abreview

  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

    Bottom blot (B): As above, except ab6982 omitted.

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
  • Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
    Functional Studies - Anti-Adenovirus Type 5 antibody (ab6982)
    Additional information regarding Adenovirus type 5 capsid proteins. 
  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview
    All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    All lanes : human hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocked with 5% milk

    See Abreview

  • Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
    Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : Whole cell lysate prepared from U2OS cells.
    Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit conjugated to IRDye 700DX


    Blocking step was performed using 5% milk for 1 hour at 23°C.

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (59)

    ab6982 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6982 は 59 報の論文で使用されています。

    • Wang Z  et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638
    • Brugada-Vilà P  et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics 10:2744-2758 (2020). PubMed: 32194832
    • Saari H  et al. Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. J Extracell Vesicles 9:1747206 (2020). PubMed: 32363012
    • Costa R  et al. Characterization of Adenovirus 5 E1A Exon 1 Deletion Mutants in the Viral Replicative Cycle. Viruses 12:N/A (2020). PubMed: 32075072
    • Crisostomo L  et al. Temporal dynamics of adenovirus 5 gene expression in normal human cells. PLoS One 14:e0211192 (2019). PubMed: 30677073
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 29 Abreviews or Q&A

    Western blot abreview for Anti-Adenovirus Type 5 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    infected with Adv-iNOS
    Specification
    human hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 31 2017

    Question

    I would like to buy your anti-adenovirus type 5 antibody (ab6982) and am wondering if your antibody can target Ad type 5 knob domain??? Could you check it for me?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 16 2013

    Answer


    ab6982 is a plyclonal antibody and as such shows recognition to every protein in the Ad5 capsid, including the knob.

    Read More

    Abcam Scientific Support

    Answered on Jan 16 2013

    Question

    It may be possible to reduce the cross-reactive signal by alterring some steps of the protocol. Could you tell exactly in wich step please?

    At what dilution are you using the antibody?1/100

    What kind of blocking solution do you use? Normal Horse Serum 1/5 in PBS 1% BSA for 1-2 hours at RT

    Could you describe the antigen retrieval protocol that you use? 10-15' heating plus 2' boiling in a presurized bath in Citrate Sodium Solution pH6; 20' cooling slices

    Thanks a lot!

    Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2012

    Answer

    Thank you very much for your reply.

    If the specific signal is fairly strong, you can further dilute the antibody to 1:200-1:1000 which should help reduce non-specific binding. You could also try blocking the tissue overnight at 4C, and continue to use the 1:5 serum for blocking.

    We do fully guarantee this antibody to work in IHC-P, so if these suggestions aren't helpful then I'd be happy to send a replacement with our other adenovirus type 5 antibody, ab3165, which is a monoclonal and may be more specific-

    https://www.abcam.com/Adenovirus-Type-5-antibody-M58-ab3165.html

    We can alternatively issue a credit or refund for your original purchase. Please keep me updated about any new results with ab6982, and let me know if you'd like to receive a replacement, credit, or refund. I look forward to hearing from you. If there is anything else that we can do for you please don't hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2012

    Question

    I'm using Ab6982 in parafined tissues immunohistochemistry and I get lot of crossreacting signal, with canine eritrocites and other unspecific tissues.

    Do you have some kind of explanation or knowledge about that? Any other crossreacting signal Known?



    Thanks a lot for your help!

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab to request more information about this antibody.

    The antibody was validated in IHC according to the following publication, and unfortunately we haven't tested this antibody in IHC in our own labs so we haven't seen this kind of cross-reactivity-
    Guedan S et al. Hyaluronidase expression by an oncolytic adenovirus enhances its intratumoral spread and suppresses tumor growth. Mol Ther 18:1275-83 (2010).
    The immunogen was a pharmaceutical grade purified virus so we expected minimal cross-species recognition. The virus was grown in mammalian cells (HEK 293) so it's possible that there may be some cross recognition to other mammalian targets, but unfortunately we do not know what specific proteins the antibody would cross-react with.
    It may be possible to reduce the cross-reactive signal by alterring some steps of the protocol. At what dilution are you using the antibody? What kind of blocking solution do you use? Could you describe the antigen retrieval protocol that you use?
    I look forward to hearing from you. We do fully guarantee this antibody to work in IHC on paraffin-embedded tissue for up to 6 months after purchase, so I can issue a replacement, credit, or refund if you are not satisfied with the results. If you would like to accept one of these options, please let me know your original order or PO number and I'll set this up promptly.
    Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Oct 17 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Adenovirus Type 5 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (AdV-infected submandibular salivary gland)
    Specification
    AdV-infected submandibular salivary gland
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - tritonX 0.2%
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 02 2012

    Western blot abreview for Anti-Adenovirus Type 5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hek293)
    Loading amount
    25 µg
    Specification
    Hek293
    Treatment
    AdV-infected
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Tris-Bis)
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 02 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Adenovirus Type 5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 15°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 24 2012

    Question

    Our customer would like to purchase adenovirus antibody which reacts to serotype 5.
    I searched ab6982 but I'm not sure type 5 and serotype 5 are the same mean.

    Specificity
    Targets all capsid proteins of Adenovirus type 5, including hexon, fiber and penton. Cross reacts with other serotypes of Adenovirus.

    Please let me know if it is right one.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Thank you for contacting us.

    Yes, I can confirm that "Type 5" is a shortened version of the more scientifically precise term serotype 5. They both mean exactly the same.All Adenovirus classification is based upon serotyping. This antibody ab6982,was raised against serotype 5 Adenovirus.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 19 2012

    Question

    do you have anti-adenovirus antibody which have the same specificity as ab6982 but biotin-labelled?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2012

    Answer

    Thank you for contacting us.

    We do not have this antibody inbiotinylated form, but we do have conjugation kits for biotinylation:

    https://www.abcam.com/easylink

    Biotin type A (e.g. ab102865 (3x10 ug) and other sizes)
    https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10µg-Biotin-ab102865.html

    Biotin type B (e.g. ab102868 (3x10 ug) and other sizes)
    https://www.abcam.com/EasyLink-Biotin-type-B-Conjugation-Kit-3-x-10µg-Biotin-ab102868.html

    Since the antibody comes as whole antiserum, it would need to be purified before the conjugation reaction, using an Antibody serum purification kit (1 (ab109208) or 3 purifications(ab109209)).

    I will also check with the lab if a custome conjugation might be possible, but if it would be possible, it would require an orderof at least 10 vials. I will let you know what I find out.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 13 2012

    Question

    Phone call: Customer kindly asked for the actual concentration of anti-Adenovirus Type 5 antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 28 2011

    Answer

    Thank very much for your patience. This antibody has been developed as a serum; it is not purified further. We are unable to provide the purified version of this product. We also can’t purify it as a custom request. I am sorry we are unable to fulfil your request this time. We are however ready to provide any additional information you may need which can help you to develop your experiment. I hope this information will be helpful. Should you any further questions please do not hesitate to ask?

    Read More

    Abcam Scientific Support

    Answered on Dec 28 2011

    1-10 of 29 Abreviews or Q&A

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