Anti-Adenosine Receptor A2a 抗体 (ab3461)
Key features and details
- Rabbit polyclonal to Adenosine Receptor A2a
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Adenosine Receptor A2a antibody
Adenosine Receptor A2a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Adenosine Receptor A2a -
由来種
Rabbit -
特異性
Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Dog, Human
交差が予測される動物種: Horse -
免疫原
Synthetic peptide corresponding to Dog Adenosine Receptor A2a aa 373-391.
Sequence:ESHGDMGLPDVELLSHELK
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC | Use at an assay dependent concentration. | |
WB | 1/1000. | |
IHC-P | 1/20 - 1/200. |
ターゲット情報
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機能
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
ドメイン
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface. -
翻訳後修飾
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: Q6TLI7 Horse
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
see all -
別名
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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All lanes : Anti-Adenosine Receptor A2a antibody (ab3461) at 1/500 dilution
Lane 1 : Human placenta cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Mouse liver cell lysate
Lysates/proteins at 25 µg per lane. -
Immunocytochemistry/Immunofluorescence analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3461 in 3% BSA-PBS at a dilution of 1:20 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)This image is courtesy of an anonymous AbreviewAnti-Adenosine Receptor A2a antibody (ab3461) at 1/1000 dilution + Human heart tissue lysate at 20 µg
Secondary
Donkey HRP-conjugate anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
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ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
参考文献 (19)
ab3461 は 19 報の論文で使用されています。
- Castro CM et al. Adenosine A2A receptor (A2AR) stimulation enhances mitochondrial metabolism and mitigates reactive oxygen species-mediated mitochondrial injury. FASEB J 34:5027-5045 (2020). PubMed: 32052890
- Schröder S et al. PET Imaging of the Adenosine A2A Receptor in the Rotenone-Based Mouse Model of Parkinson's Disease with [18F]FESCH Synthesized by a Simplified Two-Step One-Pot Radiolabeling Strategy. Molecules 25:N/A (2020). PubMed: 32252340
- Xu K et al. Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). J Leukoc Biol 105:1225-1234 (2019). PubMed: 30907983
- Han YY et al. Cordycepin improves behavioral-LTP and dendritic structure in hippocampal CA1 area of rats. J Neurochem 151:79-90 (2019). PubMed: 31314908
- Gordian E et al. Transforming growth factor ß-induced epithelial-to-mesenchymal signature predicts metastasis-free survival in non-small cell lung cancer. Oncotarget 10:810-824 (2019). PubMed: 30783512