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    adenosine-receptor-a2a-antibody-ab3461.pdf

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Anti-Adenosine Receptor A2a 抗体 (ab3461)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (27)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
  • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

Key features and details

  • Rabbit polyclonal to Adenosine Receptor A2a
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Adenosine Receptor A2a antibody [EPR23731-50] (ab260032)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Adenosine Receptor A2a antibody
    Adenosine Receptor A2a 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Adenosine Receptor A2a
  • 由来種

    Rabbit
  • 特異性

    Detects adenosine receptor A2a. This antibody does not detect other AR subtypes.
  • アプリケーション

    適用あり: ICC, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Horse
  • 免疫原

    Synthetic peptide corresponding to Dog Adenosine Receptor A2a aa 373-391.
    Sequence:

    ESHGDMGLPDVELLSHELK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • IHC-P: Human testis and placenta tissues. ICC: U251 and SKNSH cells. WB: HepG2 and HeLa cell lysates; Human placenta tissue lysate; Mouse liver tissue lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Adenosine Receptor A2a protein (ab126024)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3461の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
WB (1)
1/1000.
IHC-P
1/20 - 1/200.
特記事項
ICC
Use at an assay dependent concentration.
WB
1/1000.
IHC-P
1/20 - 1/200.

ターゲット情報

  • 機能

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.
  • ドメイン

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • 翻訳後修飾

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P29274 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 135 Human
    • Entrez Gene: 11540 Mouse
    • Omim: 102776 Human
    • SwissProt: Q6TLI7 Horse
    • SwissProt: P29274 Human
    • SwissProt: Q60613 Mouse
    • Unigene: 197029 Human
    • Unigene: 333734 Mouse
    • 別名

      • A2AAR antibody
      • A2aR antibody
      • AA2AR_HUMAN antibody
      • ADENO antibody
      • Adenosine A2 receptor antibody
      • Adenosine A2a receptor antibody
      • Adenosine receptor A2a antibody
      • Adenosine receptor subtype A2a antibody
      • ADORA 2 antibody
      • ADORA2 antibody
      • ADORA2A antibody
      • hA2aR antibody
      • RDC 8 antibody
      • RDC8 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
      Western blot - Anti-Adenosine Receptor A2a antibody (ab3461)
      All lanes : Anti-Adenosine Receptor A2a antibody (ab3461) at 1/500 dilution

      Lane 1 : Human placenta cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Mouse liver cell lysate

      Lysates/proteins at 25 µg per lane.
    • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)

      Immunocytochemistry/Immunofluorescence analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3461 in 3% BSA-PBS at a dilution of 1:20 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (ab3461)

      ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (ab3461)
      ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (27)

    ab3461 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3461 は 27 報の論文で使用されています。

    • Li D  et al. Gut microbiota-derived inosine from dietary barley leaf supplementation attenuates colitis through PPAR? signaling activation. Microbiome 9:83 (2021). PubMed: 33820558
    • Yang D  et al. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway. Elife 10:N/A (2021). PubMed: 33416495
    • Jin H  et al. Increased Extracellular Adenosine in Radiotherapy-Resistant Breast Cancer Cells Enhances Tumor Progression through A2AR-Akt-ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33925516
    • Bhalla M  et al. A1 adenosine receptor signaling reduces Streptococcus pneumoniae adherence to pulmonary epithelial cells by targeting expression of platelet-activating factor receptor. Cell Microbiol 22:e13141 (2020). PubMed: 31709673
    • Lisztes E  et al. Adenosine Promotes Human Hair Growth and Inhibits Catagen Transition In Vitro: Role of the Outer Root Sheath Keratinocytes. J Invest Dermatol 140:1085-1088.e6 (2020). PubMed: 31730764
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    ELISA abreview for Anti-Adenosine Receptor A2a antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Cell (Cell Membrane)
    Specification
    Cell Membrane
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Tammy Zhai

    Verified customer

    投稿 Apr 22 2015

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 10 2013

    Question

      A researcher from Mexico needs to use a Anti-Adenosine Receptor A2a antibody in rat and CoIP. I found ab3461 but it has not been tested for rat. Could you check the homology to see if you would recommend trying this antibody and if the 100% testing discount would apply. He also needs Anti-Dopamine D2 Receptor antibody to use in rat and CoIP. I found ab21218 that has been tested in rat and IP, but he wants to know if it will work in CoIP as well.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Hi Daniela, I did an alignment between the rat sequence and the immnunogen sequence for ab3461 and I would not recommend using this antibody in rat. The level of similarity between the two is to low. I would however recommend ab101678, it has not been tested in IP, but it will react with rat tissue. The customer could get a testing discount for trying it out in IP. As for ab21218, I think that one should work in CoIP, if it works in IP, it should work for CoIP. Let me know if there is anything else I can help with.  

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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