Anti-ADAMTS5 抗体 (ab231595)
Key features and details
- Rabbit polyclonal to ADAMTS5
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
製品の概要
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製品名
Anti-ADAMTS5 antibody
ADAMTS5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAMTS5 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Rat ADAMTS5 aa 250-650. (Expressed in E.coli).
Database link: Q6TY19 -
ポジティブ・コントロール
- WB: Human A549 and HeLa cell lysates; recombinant rat ADAMTS5 protein. IHC-P: Ovary tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231595の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 102 kDa.
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特記事項 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 102 kDa. |
ターゲット情報
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機能
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
組織特異性
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
ドメイン
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 11096 Human
- Entrez Gene: 23794 Mouse
- Entrez Gene: 304135 Rat
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- SwissProt: Q9R001 Mouse
- Unigene: 58324 Human
- Unigene: 112933 Mouse
see all -
別名
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
画像
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Formalin-fixed, paraffin-embedded rat ovary tissue stained for ADAMST5 using ab231595 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-ADAMTS5 antibody (ab231595) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 102 kDa -
Anti-ADAMTS5 antibody (ab231595) at 2 µg/ml + A549 (Human lung carcinoma cell line) cell lysate
Predicted band size: 102 kDa -
Anti-ADAMTS5 antibody (ab231595) at 2 µg/ml + Recombinant rat ADAMTS5 protein
Predicted band size: 102 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab231595 は 1 報の論文で使用されています。
- Wang T et al. Long non-coding RNA XIST promotes extracellular matrix degradation by functioning as a competing endogenous RNA of miR-1277-5p in osteoarthritis. Int J Mol Med 44:630-642 (2019). PubMed: 31198977