Application
Western blot
Sample
Human Cell lysate - whole cell (4L melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
4L melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
6 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Praveen Agrawal
Verified customer
投稿 Mar 24 2017