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  1. Link

    adam10-antibody-ab1997.pdf

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Cell Biology Apoptosis Receptors Receptor Processing
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Anti-ADAM10 抗体 (ab1997)

  • Datasheet
  • SDS
Reviews (24)Q&A (4)References (94)

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Western blot - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Western blot - Anti-ADAM10 antibody (ab1997)
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
  • Western blot - Anti-ADAM10 antibody (ab1997)

Key features and details

  • Rabbit polyclonal to ADAM10
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-ADAM10 antibody [EPR5622] (ab124695)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-ADAM10 antibody
    ADAM10 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to ADAM10
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICCmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human ADAM10 aa 732-748.
    Sequence:

    PQRQRPRESYQMGHMRR


    (Peptide available as ab7868)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • ADAM Protein Family
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • ADAM protein family
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ADAMs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Neuroscience
    • Diseases

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • ADAM10 peptide (ab7868)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ADAM10 protein (ab82822)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1997の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (10)
1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ICC (1)
Use a concentration of 2 µg/ml.
特記事項
WB
1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ICC
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • 組織特異性

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • 配列類似性

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • ドメイン

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾

    The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Target information above from: UniProt accession O14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 282132 Cow
    • Entrez Gene: 102 Human
    • Entrez Gene: 397345 Pig
    • Entrez Gene: 29650 Rat
    • Omim: 602192 Human
    • SwissProt: Q10741 Cow
    • SwissProt: O14672 Human
    • SwissProt: O77633 Pig
    • SwissProt: Q10743 Rat
    • Unigene: 578508 Human
    • Unigene: 42924 Rat
    see all
  • 別名

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

画像

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)
    Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
    Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml

    All lanes : Jurkat whole cell lysate

    Predicted band size: 84 kDa

  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    ab1997 at 2µg/ml staining K562 cells by ICC/IF.
  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an anonymous Abreview
    All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution

    Lane 2 : ADAM10 siRNA treated human keratinocyte cells
    Lane 3 : Non-treated human keratinocyte cells.

    Secondary
    All lanes : HRP conjugated Goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 100,75 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunocytochemistry - Anti-ADAM10 antibody (ab1997)
    Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.

  • Western blot - Anti-ADAM10 antibody (ab1997)
    Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an Abreview submitted by Grant Corbett
    All lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution

    All lanes : Human frontal cortex tissue lysate - whole

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 65,80 kDa why is the actual band size different from the predicted?


    Exposure time: 330 seconds

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (94)

ab1997 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1997 は 94 報の論文で使用されています。

  • Cheng Y  et al. ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc. Cancer Cell Int 21:32 (2021). PubMed: 33413403
  • Diaz-Lucena D  et al. TREM2 expression in the brain and biological fluids in prion diseases. Acta Neuropathol 141:841-859 (2021). PubMed: 33881612
  • Ni XQ  et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY) 13:5164-5184 (2021). PubMed: 33535178
  • Pichler R  et al. Expression of ADAM Proteases in Bladder Cancer Patients with BCG Failure: A Pilot Study. J Clin Med 10:N/A (2021). PubMed: 33672843
  • Sánchez-Del-Campo L  et al. MITF induces escape from innate immunity in melanoma. J Exp Clin Cancer Res 40:117 (2021). PubMed: 33789714
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 28 Abreviews or Q&A

Western blot abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 18 2022

Western blot abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Melanoma cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Aug 03 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Choroid plexus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Choroid plexus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 22 2021

Immunohistochemistry (Frozen sections) abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Choroid plexus)
Permeabilization
Yes - EDTA
Specification
Choroid plexus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jan 22 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Glia cells)
Permeabilization
No
Specification
Glia cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More

MRS. Simone Grad

Verified customer

投稿 Oct 28 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jul 10 2020

Immunocytochemistry abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Liver cells)
Permeabilization
No
Specification
Liver cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jul 06 2020

Immunohistochemistry (Frozen sections) abreview for Anti-ADAM10 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Melanoma)
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jun 26 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH = 8
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 26 2020

Immunoprecipitation abreview for Anti-ADAM10 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (kidney)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
kidney
Read More

Dr. Nora Alghamdi

Verified customer

投稿 Nov 22 2018

1-10 of 28 Abreviews or Q&A

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