製品の概要
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製品名Anti-ADAM10 antibody
ADAM10 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ADAM10
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WB, ICC, ELISA, ICC/IFmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide corresponding to Human ADAM10 aa 732-748.
Sequence:PQRQRPRESYQMGHMRR
(Peptide available asab7868) -
ポジティブ･コントロール
- WB: Jurkat whole cell lysate. ICC: K562 cells. ICC/IF: HeLa and K562 cells.
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特記事項TNFa converting enzyme.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPBS with 0.02% sodium azide
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab1997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/500. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868). | |
| ICC | Use a concentration of 2 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 2 µg/ml. |
ターゲット情報
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機能Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. -
組織特異性Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
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配列類似性Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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翻訳後修飾The precursor is cleaved by a furin endopeptidase.
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細胞内局在Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
- Information by UniProt
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参照データベース
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Omim: 602192 Human
- SwissProt: O14672 Human
- SwissProt: O35598 Mouse
- Unigene: 578508 Human
- Unigene: 3037 Mouse
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別名
- A disintegrin and metalloprotease domain 10 antibody
- A disintegrin and metalloproteinase domain 10 antibody
- AD 10 antibody
see all
画像
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Western blot - Anti-ADAM10 antibody (ab1997)Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml
All lanes : Jurkat whole cell lysate
Predicted band size: 84 kDa -
Immunocytochemistry - Anti-ADAM10 antibody (ab1997)ab1997 at 2µg/ml staining K562 cells by ICC/IF. -
Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (ab1997)ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an anonymous AbreviewAll lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution
Lane 2 : ADAM10 siRNA treated human keratinocyte cells
Lane 3 : Non-treated human keratinocyte cells.
Secondary
All lanes : HRP conjugated Goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100,75 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (ab1997)Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml. -
Western blot - Anti-ADAM10 antibody (ab1997)This image is courtesy of an Abreview submitted by Grant CorbettAll lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution
All lanes : Human frontal cortex tissue lysate - whole
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 65,80 kDa (why is the actual band size different from the predicted?)
Exposure time: 330 seconds
プロトコール
参考文献
This product has been referenced in:
- Linsenmeier L et al. Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein. Mol Neurodegener 13:18 (2018). Mouse . Read more (PubMed: 29625583) »
- Tosetti F et al. Specific ADAM10 inhibitors localize in exosome-like vesicles released by Hodgkin lymphoma and stromal cells and prevent sheddase activity carried to bystander cells. Oncoimmunology 7:e1421889 (2018). Read more (PubMed: 29721369) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"