Application
Western blot
Sample
Human Cell lysate - whole cell (colon cancer cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
chemical inhibitor
Specification
colon cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: invitrogen goat anti-rabbit IgG H+L
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 35 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 19 2020