Anti-Caspase-3 抗体 (ab49822)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Caspase-3 antibody
Caspase-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-3 -
由来種
Rabbit -
特異性
The immunogen for this product is aa167-175 within the p17 subunit (cleaved caspase) but because this is a poly the epitope will vary and not all batches will be flush against the cleavage site.
This means it will detect a number of additional cleavage products in addition to p17:
• P12+17,
• P12+P17+propeptide 1
• p12+p17+proppetide 1 + propeptide 2,
• P17+propeptide 1,
• p17+propeptide 1 + propeptide 2 -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-3 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: P42574 -
ポジティブ・コントロール
- ICC/IF: Human epitheloid carcinoma HeLa cell line, treated with staurosporine and WB: Human recombinant caspase-3, active.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Whole antiserum is fractionated and then further purified by ion exchange chromatography. The resulting IgG fraction is further purified by absorption on the Caspase-3 peptide (Human, amino acids 167-178) corresponding to the uncleaved Caspase 3 site sequence. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/500.
|
ICC/IF |
1/1000.
|
特記事項 |
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WB
1/500. |
ICC/IF
1/1000. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
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別名
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
画像
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Anti-Caspase-3 antibody (ab49822) at 1/1000 dilution
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cleaved Caspase-3 with ab49822 at 1/1000. HeLa cells were treated with 1 μg/mL staurosporine, fixed and permeabilized with Methanol followed by Acetone. Goat Anti-Rabbit IgG (whole molecule)-FITC was used as the secondary antibody. Cells were counterstained with DAPI (blue) to stain nuclei.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (388)
ab49822 は 388 報の論文で使用されています。
- Song BW et al. Cold-pressed oil from Citrus aurantifolia inhibits the proliferation of vascular smooth muscle cells via regulation of PI3K/MAPK signaling pathways. Exp Ther Med 23:21 (2022). PubMed: 34815773
- Dai HT et al. microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2. Neoplasma 69:16-27 (2022). PubMed: 34818025
- Jiang J et al. Hyperoxia induces alveolar epithelial cell apoptosis by regulating mitochondrial function through small mothers against decapentaplegic 3 (SMAD3) and extracellular signal-regulated kinase 1/2 (ERK1/2). Bioengineered 13:242-252 (2022). PubMed: 34898379
- Wu Q et al. A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats. J Mol Neurosci 72:730-740 (2022). PubMed: 34988900
- Sahyon HAE et al. Anti-proliferative effects of the combination of Sulfamethoxazole and Quercetin via caspase3 and NFkB gene regulation: an in vitro and in vivo study. Naunyn Schmiedebergs Arch Pharmacol 395:227-246 (2022). PubMed: 34994822